硒拮抗氟对人肝细胞DNA损伤及凋亡的影响

Effects of selenium against fluoride on DNA damage and apoptosis of human hepatocytes

摘要:
目的研究氟、硒对离体人肝细胞DNA损伤及凋亡的作用。
方法体外培养的人肝细胞分别接触一定剂量的氟和/或硒12h后, 检测肝细胞DNA的损伤率、凋亡率和细胞周期构成比。
结果氟组肝细胞DNA损伤率、凋亡率和S期细胞数均明显高于对照组和氟+硒组(P < 0.05), 硒组DNA损伤率和凋亡小体育分率均高于对照组, 但无显著性差异(P > 0.05).
结论一定剂量的硒可拮抗氟对离体人肝细胞DNA损伤及诱导的肝细胞凋亡作用。

Abstract:
Objective To investigate the influence of selenium and fluoride on DNA damage and apoptosis in human hepatocytes in vitro.
Methods The DNA damage rate, cellular apoptosis, and cell cycle in human hepatocytes were measured after hepatocytes were incubated with fluoride and/or selenium for 12 hours in vitro.
Results The DNA damage rate, apoptosis, and cell number in Sphase in hepatocytes in the fluoride group were significantly higher than the control group and the fluoride-exposed and selenium-treated groups(P < 0.05).The DNA damage rate and apoptosis in hepatocytes in the selenium group were higher than control group, but there was no significant difference between selenium group and control group(P > 0.05).
Conclusion Some dose of selenium can antagonize DNA damage and apoptosis in hepatocytes induced by fluoride.