葡多酚对大鼠肝PARP mRNA异常表达的抑制

丛红群; 钟进义

葡多酚对大鼠肝PARP mRNA异常表达的抑制

Inhibitory effect of grape procyanidin on abnormal expression of parp mRNA in rat's hepatic cells

摘要

摘要:
目的研究葡多酚(GPC)对N-亚硝基化合物引发的大鼠肝细胞聚腺苷二磷酸核糖聚合酶〔(poly(ADP-ribose)polymerase, PARP)〕mRNA表达的影响作用。
方法采用0.5%的亚硝酸钠经口灌胃诱发大鼠肝脏突变, 2个GPC实验组同时经口分别给予GPC 100和10 mg/kg, 8周后体内灌注固定肝组织, 用原位杂交技术检测肝细胞PARP mRNA的表达水平, 用图像分析仪对其表达水平进行定量分析。
结果诱变损伤组PARP mRNA表达的阳性细胞率和吸光度分别为30.12%和0.3543±0.0419, 高剂量GPC组则分别为11.48%和0.2687±0.0172, 两组之间均有显著性差异(P < 0.01)。
结论葡多酚可抑制N-亚硝基化合物引发的大鼠肝细胞PARP mRNA的异常表达。

Abstract:
Objective To study the effect of grape procyanidin(GPC)on N-nitroso compounds-induced expression of poly(ADP-ribose)polymerase(PARP)mRNA in rat's hepatic cells.
Methods 0.5% NaNO₂ was infused into Wistar rats to induce hepatocytes.mutation, and GPC was infused by 100 and 10 mg/kg respectively through mouth of the two experimental groups at the same time.Aftereight weeks, hepatic tissue was fixed in vivo and situ hybridization was applied to measure the expression level of PARP mRNA in rats.hepatic cells, and its expression level was quantitatively analyzed by an image analysis instrument.
Results The positive cells rate of PARP mRNA expressed in the induced-injury group was 30.12% and optical density was 0.354 3±0.041 9, and those of the high-dose GPC group were 11.48% and 0.268 7± 0.017 2, and the difference between the two groups had statistical significance(P < 0.01).
Conclusion GPC had inhibitory effect on N-nitroso compounds-induced abnormal expression of PARP mRNA in hepatic cells of rats.

HTML全文

参考文献(10)

施引文献

资源附件(0)