Abstract:
Objective To search for the mechanism of tea polyphenols on cardiovascular diseases, the expressions of NADPH oxidase subuints in endothelial cells were observed.
Methods Tea polyphenols were co-incubated with bovine carotid artery endothelial cells(BCA ECs)in vitro and the intracellular NADPH oxidase subunits p22phox and p67phox proteins were detected using western blotting method.
Results Both 0.4 and 4.0μg/ml tea polyphenols(either green tea or black tea)down-regulated NADPH oxidase p22phox and p67phox expressions in a dose-negative relationship(P < 0.05).
Conclusion Tea polyphenols can regulate the enzyme that played an important role in the process of ROS production in endothelial cells, which may be benefical for the prevention of endothelial cell dysfunction and the development of cardiovascular diseases.
下载: