麻疹病毒血凝蛋白基因的序列分析

Sequence analysis on hemagglutinin gene of mealses virus isolated in Shenzhen

摘要:
目的探讨深圳市麻疹病毒的基因型别和特征。
方法对深圳市专科医院2000~2002年的19例临床诊断为麻疹病例的咽拭子和尿液提取RNA, 通过RTPCR方法扩增麻疹病毒血凝素(H)基因序列, 并克隆到PMD18Tvector进行序列测定; 分析麻疹病毒和Genebank中麻病毒的各基因型代表株序列的同源性。
结果 19株麻疹病毒H基因之间的同源性为9.62%~100%, 和H₁基因型代表株相比同源性为9.64%~99.3%。
结论深圳市麻疹流行株属于H₁基因型。

Abstract:
Objective To explore the genetic characterization and genotype of measles viruses circulating in Shenzhen city during the year of 2000-2002.
Methods Extracting RNA from throat swabs and urine of the measles patients, using RT-PCR amplification measles virus hemagglutinin gene of 571bp.PCR products were cloned into PM D18-T vector and the homogeneity of the virus isolated were compared with the strains in genebank.
Results H genes homogeneity was between the range of 96.2%-100% compared with other wild-type viruses of H₁ genoty pe, the homogeneity was 96.4%-99.3%.
Conclusion Shenzhen circulating measles viruses were belong ed to the clade H₁.