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刘艺敏, 张敬, 朱华凤, 封蕾. 维生素对肺泡巨噬细胞NOS活性的影响[J]. 中国公共卫生, 2004, 20(3): 295-296.
引用本文: 刘艺敏, 张敬, 朱华凤, 封蕾. 维生素对肺泡巨噬细胞NOS活性的影响[J]. 中国公共卫生, 2004, 20(3): 295-296.
shu
LIU Yi-min, ZHANG Jing, ZHU Hua-feng, . Effects of vitamin on nitric oxide synthase activity of alveolar macrophage[J]. Chinese Journal of Public Health, 2004, 20(3): 295-296.
Citation: LIU Yi-min, ZHANG Jing, ZHU Hua-feng, . Effects of vitamin on nitric oxide synthase activity of alveolar macrophage[J]. Chinese Journal of Public Health, 2004, 20(3): 295-296.
shu

维生素对肺泡巨噬细胞NOS活性的影响

Effects of vitamin on nitric oxide synthase activity of alveolar macrophage

    摘要
  • 摘要:
      目的   研究维生素E(VE)和维生素C(VC)拮抗石英粉尘细胞毒的作用及机制。
      方法   采用体外细胞培养法测定在不同浓度的VE和VC的共同作用下, 体外染石英尘肺泡巨噬细胞(AM)的存活率和细胞活力, 同时观察细胞内一氧化氮和活性氧的产生以及一氧化氮合酶活性的变化。
      结果   在体外染石英尘肺泡巨噬细胞中加入不同浓度的VE和VC后可提高该细胞的存活率, 降低NOS的活性, 减少NO和活性氧的产生, 尤以100μmol/L VE和100μmol/L VC作用组效果最为明显。
      结论   VE和VC具有拮抗石英尘对肺泡巨噬细胞的细胞毒作用, 具有保护AM的作用。

     

    Abstract:
      Objective   In order to study the effects of vitamin E and vitamin C on the injury of alveolar macrophages(AM) exposed to quartz in vitro.
      Methods   Different dosages of vitamin E and vitamin C were respectively added into AM medium, cultured with exposure of quartz in vitro.Cell activities of the three groups were observed.Also nitric oxide(NO) and the activity of nitric oxide synthase(NOS) in AM were carried out.
      Results   Vitamin E and vitamin C could increase survival rate and the activity of AM.At the same time, activities of NO and NOS were decreased, especially the combination of VE and VC both at 100 μmol/L consistency, having the dramatic inhibitory effect.
      Conclusion   VE and VC can inhibit the activity of NOS, decrease the amount of NO, antagonize the injury of AM exposed to quartz.

     

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