氯仿抽提细胞培养物中纯化甲肝病毒

Investigation on factors affecting purification of HAV with chloroform

摘要:
目的探讨氯仿抽提纯化甲型肝炎病毒(HAV)过程中多种因素对其活性的影响。
方法利用正交分析方法, 对抽提纯化过程中超声波、氯仿、洗涤液等多种因素进行系统的比较。
结果病毒液的体积与超声波功率相匹配破碎效果较好; 用PBS及NS作洗涤液比MEM效果好; 氯仿的质量也是影响抽提效果的重要因素。
结论氯仿抽提纯化HAV过程中多种因素均会对其活性产生影响, 需探索一个合适条件, 最大限度地降低对其活性的影响。

Abstract:
Objective To investigate the factors relating to the purification of HAV.
Methods It was performed by the orthogonal experiment method to analyze some factors relating to the purification of HAV with chloroform.
Results The matches between the powers of sonication and the sample volumes of virus were important factors in the titration of virus.It was better to use PBS and NS as washing buffer than MEM.The quality of chloroform also influenced the purification of HAV.
Conclusion The purification of HAV was influenced by several factors, and it was necessary to optimize these factors to reduce the influence on the activity of HAV.