微波消解法测定胎儿大脑组织中生物必需元素

刘小立; 谢建滨; 刘桂华; 王继尧; 柳其芳

微波消解法测定胎儿大脑组织中生物必需元素

Study on determination of K、Na、Mg、P、Cu、Fe、Mn、Zn in fetal cerebra by microware dissolution and inductively couple plasma-AES spectrometry

摘要

摘要:
目的建立胎儿大脑组织中生物必需元素K、Na、Mg、P、Cu、Fe、Mn、Zn的分析方法。
方法样品经HNO₃+H₂O₂微波消解体系消解后, 用电感耦合等离子体发射光谱法(ICP-AES)同时测定K、Na、Mg、P、Cu、Fe、Mn、Zn含量。
结果元素的检出限分别在0.007mg/L~0.1mg/L之间; 线性良好, 线性相关系统均≥0.999;重现性好, 变异系统RSD<2%;准确度高, 回收率在93.7%~16.1%之间, 用猪肝(GBW08551)及人发(GBW07601)标样质控, 分析结果与标准值吻合。
结论本法快速灵敏, 精密度及准确度均符合要求, 结果令人满意。

Abstract:
Objective To establish the method for the determination of biological essential elements K、Na、Mg、P、Cu、Fe、Mn、Zn in Fetal Cerebra.
Methods The samples were digested by closed-vessel microwave HNO₃+H₂O₂ dissolution system.The solution was directly analyzed by ICP-AES for the determination of K、Na、Mg、P、Cu、Fe、Mn、Zn.
Results The detection limits of each element was between 0.007 mg/L and 0.1 mg/L.Standard linear comelationr≥0.999.Standard deviations were below 2%.Recoveries of spiked saniples were of the range 93.7% and 116.1%.Two standard reference materials nanlely "pig Liver GBW08551" and "Human Hair GBW07601" were used an quality control standards and the analysis results conformed well to the certified values.
Conclusion The method was proved to be simple, rapid, accurate and reliable.And it has been applied to the analysis of the sample with satisfactory results.

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