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李小燕, 陈贤均. 溴虫腈对小鼠脾细胞和肝细胞DNA损伤作用[J]. 中国公共卫生, 2004, 20(3): 320-322.
引用本文: 李小燕, 陈贤均. 溴虫腈对小鼠脾细胞和肝细胞DNA损伤作用[J]. 中国公共卫生, 2004, 20(3): 320-322.
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LI Xiao-yan, CHEN Xian-jun. Effects of chlorfenapyr on DNA damage of spleen cells and liver cells in mice by single cell gel electrophoresis[J]. Chinese Journal of Public Health, 2004, 20(3): 320-322.
Citation: LI Xiao-yan, CHEN Xian-jun. Effects of chlorfenapyr on DNA damage of spleen cells and liver cells in mice by single cell gel electrophoresis[J]. Chinese Journal of Public Health, 2004, 20(3): 320-322.
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溴虫腈对小鼠脾细胞和肝细胞DNA损伤作用

Effects of chlorfenapyr on DNA damage of spleen cells and liver cells in mice by single cell gel electrophoresis

    摘要
  • 摘要:
      目的   探讨溴虫腈对小鼠脾细胞和肝细胞DNA的损伤作用。
      方法   将溴虫腈按4.9, 9.8及19.6mg/kg3个不同剂量, 一次性灌胃染毒小鼠, 24h后用单细胞凝胶电泳技术检测小鼠脾、肝细胞的拖尾率和DNA迁移距离。
      结果   脾细胞3个剂量组拖尾细胞率和DNA迁移距离分别是对照组的4.8~17.6倍(P < 0.001)和1.6~2.9倍(P < 0.05~0.01);肝细胞3个剂量组的拖尾细胞率和DNA迁移距离分别是对照组的7.5~10.4倍(P < 0.001)和20~28倍(P < 0.01~0.001);且表现出明显的剂量效应关系(3者的r值依次分别为0.995, 0.9987和0.9999, P < 0.05);在相同剂量下, 肝细胞拖尾细胞率比脾细胞均明显增高(P < 0.01);在4.9mg/kg剂量水平, 肝细胞的DNA迁移距离比脾细胞的DNA迁移距离也明显增高(P < 0.05);而在9.8和19.6mg/kg剂量水平, 2种细胞的DNA迁移距离无显著性差异(P > 0.05)。
      结论   溴虫腈能引起小鼠脾脏和肝脏细胞DNA的损伤, 且对肝细胞DNA损伤更严重。

     

    Abstract:
      Objective   To study the effects of Chlorfenapyr on DNA damage of spleen and liver cells in mice.
      Methods   Chlorfenapyr was administrated to mice with 4.9, 9.8 and 19.6 mg/kg doses once by gavage, then the percentage of cells with comets and the DNA migration of spleen cells and liver cells were detected by single cell gel electrophoresis.
      Results   Compared with the control group, the percentage of cells with comets and the DNA migration were increased significantly at all the three tested doses of the two kinds of tissue cells(P < 0.05-0.001);except for the DNA migration of spleen cells, the percentage of cells with comets of spleen cells and liver cells and the DNA migration of liver cells exhibited a significant linear dose-dependent increase(r values were 0.995, 0.9987 and 0.9999 respectively, P < 0.05);at the identical dosage criterion, the percentage of cells with comets of liver cells increased statistically at all the three tested doses as compared to the corresponding dose spleen cells groups(P < 0.01), so did the DNA migration of the 4.9 mg/kg dose of liver cells(P < 0.05).
      Conclusion   Chlorfenapyr can induce the DNA damage of spleen cells and liver cells, and the effects on the DNA damage of liver cells is stronger than that of spleen cells.

     

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