烹调油烟冷凝物对细胞的氧化损伤研究

Study on oxidative damage effects of cooking oil fume conden sation on BEAS-2B cell

摘要:
目的通过测定细胞内和细胞上清液中活性氧(reactive oxygen species, ROS)水平, 以及细胞膜上ATP酶的含量, 对烹调油烟冷凝物(COFC)诱发永生化人支气管上皮细胞(BEAS-2B)产生ROS及其对细胞膜损伤进行研究。
方法采用分光光度法检测细胞外活性氧, 荧光标记细胞内活性氧及ATP酶。
结果在烹调油烟染毒的细胞内ROS的产生与ATP酶的活性降低有关。
结论 COF引起细胞膜损伤时, ROS的大量产生可能是ATP酶活性降低的主要因素, Ca²⁺-ATP酶及Mg²⁺-ATP酶对ROS敏感性强。

Abstract:
Objective The reactive oxygen species(ROS)production and A TPase in human bronchial epithelial cell line induced by cooking oil fume condensation were studied through measuring extra celluar and intracellular ROS levels including super oxide anions(₂)and hydrogen peroxides(H₂O₂), and ATPase contents.
Methods Sepectrometric method and fluorometric method were used to measure the ROS reduced by COFC.And the activities of ATPase assayed by biochemical method.
Results The ROS correlated with the decrease of contents of A TPase.
Conclusion Membrane ATPase activity was inhibited by ROS, especially Ca²⁺ATPase and Mg²⁺ATPase.