苯并(a)芘对雄性小鼠睾丸细胞周期的影响

Study on cell cycle of testis cells mouse treated by B(a)P

摘要:
目的研究苯并(a)芘对雄性小鼠睾丸细胞周期的影响.
方法采用流式细胞术研究雄性小鼠睾丸细胞周期.
结果不同剂量的苯并(a)芘可以使睾丸细胞各时相的细胞百分数发生变化, 随着苯并(a)芘染毒剂量的增加, G₀/G₁、S时相的细胞百分数明显减少, 5, 10, 20mg/kg各剂量染毒组与阴性对照组比较差异具有显著性(P < 0.01).G₂/M时相的细胞百分数逐渐增多, 各剂量组与阴性对照组比较具有显著性差异(P < 0.01).
结论苯并(a)芘可以抑制睾丸细胞的DNA合成, 引起G₂期细胞阻滞, 使细胞有丝分裂延迟.

Abstract:
Objective Effects of B(a)P on the cell cycle of testicle cells of male mice were studied.
Methods The flow cytometry was used to study the effects of B(a)P on testicle cells of male mice.
Results The different doses of B(a)P could make the testicle cell cycle change.The cells in G₀/G₁, S phase decreased with the increase of B(a)P.There were significant differences among the 5, 10, 20 mg/kg and negative control(P < 0.05).The accounts of cells in G₂/M increased and there were significant differences between experimental groups and negative control(P < 0.05).
Conclusion B(a)P can inhibit DNA synthesis of testicle cells, cause the G₂ block and delay the mitosis of testicle cells.