氟对人胚肝细胞DNA损伤及凋亡的影响

王爱国; 夏涛; 褚启龙; 刘方; 张明; 杨克敌

氟对人胚肝细胞DNA损伤及凋亡的影响

Effects of fluoride on DNA damage and apoptosis in human embryo hepatocytes

摘要

摘要:
目的探讨氟对人胚肝细胞(L-02细胞)DNA损伤及诱导凋亡的作用.
方法采用40, 80, 160μg/ml氟化钠对培养的人胚肝细胞进行染毒, 24h后用单细胞凝胶电泳技术(SCGE)和流式细胞术(FCM)检测氟化物对人胚肝细胞DNA损伤和细胞凋亡情况.
结果氟组人胚肝细胞DNA损伤率明显高于对照组, Ridit值分别为0.582, 0.815, 0.892;中、高剂量氟组人胚肝细胞凋亡百分率(9.293±1.171), (11.727±1.196)均明显高于对照组(5.343±1.620).同时, 高剂量氟组与低剂量氟组之间细胞DNA损伤率和凋亡率的差异也存在显著性差异(P < 0.05).
结论氟化物具有引起人胚肝细胞DNA损伤和细胞凋亡的作用, 并呈现一定的剂量-效应关系.

Abstract:
Objective To explore the effects of fluoride on DNA damage and apoptosis in human embryo hepatocytes.
Methods DNA damage and apoptosis were measured after human embryo hepatocytes were incubated with 40, 80, and 160 Lg/ml sodium fluoride using Single Cell Electrophosis(SCGE)and Flow Cytometry(FCM), respectively.
Results The DNA damage of human embryo hepatocytes in the fluoride gruoup was significantly higher than the control group.Ridit value were 0.582, 0.815 and 0.892 respectively.The percentage of apoptosis in the middle and high fluoride group were markedly higher than the control group.Morevoer, the rate of DNA damage and the percentage of apoptosis in high fluoride group were significantly higher than the low fluoride group.
Conclusion Fluoride exposure can cause DNA damage and apoptosis in human embryo hepatocytes, and there existed dose-effect relationship to some extent.

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