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宋淑静, 何忠平, 庄辉, 闫杰, 董庆鸣. 中国北方5城市慢性乙型肝炎患者的基因分型[J]. 中国公共卫生, 2004, 20(2): 166-167.
引用本文: 宋淑静, 何忠平, 庄辉, 闫杰, 董庆鸣. 中国北方5城市慢性乙型肝炎患者的基因分型[J]. 中国公共卫生, 2004, 20(2): 166-167.
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SONG Shu-jing, HE Zhong-ping, ZHUANG Hui, . Genotyping of patients with chronic hepatitis B in five cities of China[J]. Chinese Journal of Public Health, 2004, 20(2): 166-167.
Citation: SONG Shu-jing, HE Zhong-ping, ZHUANG Hui, . Genotyping of patients with chronic hepatitis B in five cities of China[J]. Chinese Journal of Public Health, 2004, 20(2): 166-167.
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中国北方5城市慢性乙型肝炎患者的基因分型

Genotyping of patients with chronic hepatitis B in five cities of China

    摘要
  • 摘要:
      目的   了解中国慢性乙型肝炎(CHB)患者中的基因分型情况.
      方法   根据GenBank中18株HBVS基因核苷酸序列, 设计共同的外引物及其因型特异性内引物, 对中国北方5个城市595例CHB患者血清进行基因分型, 并对A、B、C及B、C混合型各一株进行测序.
      结果   595例血清标本中, A型8例, 占1.4%;B型53例, 占8.9%;C型360例, 占60.5%;B、混合型112例, 占188%;A、C混合型14例, 占2.4%;A、B混合型15例, 占2.5%;A、B、C混合型6例, 占1.0%;未分型者27例, 占4.5%.5城市间在B、C及B、C混合型的分布上有显著性差异(P < 0.05), 4份测序结果与PCR基因分型法一致.
      结论   在5个城市CHB患者中HBV基因型以C型为主, B型次之, 并见各种混合基因型感染, 各地区在主要基因型分布上存在显著性差异.

     

    Abstract:
      Objective   To study hepatitis B virus(HBV)genotypes of patients with chronic hepatitis B in five cities of China.
      Methods   18 typical full-genomic sequences, including A, B, C, D, E and F genotypes of HBV were analyzed and aligned.Universal outer primers and type-specific inner primers were designed on the basis of conserved nucleotide sequences from pre-S1 to S region of HBV.595 serum samples collected from patients with chronic hepatites B in 5 cities of China were tested by the PCR genotyping method, 4 samples genotyped by this method as A, B, C and coinfection of B and C were selected randomly and anlayzed by the cloning and sequencing.
      Results   Of 595 serum samples, 8(1.4%)were genotype A, 53(8.9%)genotype B, 360(60.5%)genotpye C 112(18.8%)were coinfection of genotype B and C 14(2.4%)A and C, 15(2.5%)A and B 6(1.0%)A、B and C, and remaining 27(4.5%)unspecified.Distribution of gentype B、C and coinfection of genotype B and C between 5 cities was significantly different(P < 0.05).The results of sequencing were identical with that genotyped by the PCR method.
      Conclusion   Genotype C was the most frequent genotype in patients with chronic hepatitis B in China, and genotype B was the next.Coinfections of two or more than two genotypes HBV were also found.Genotype distribution in patients with chronic hepatitis B in 5 cities studied was quite different.

     

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