抗氧化剂对紫外线诱发DNA损伤的保护作用

李晶; 刘扬; 孙炜; 程梅

抗氧化剂对紫外线诱发DNA损伤的保护作用

Protective effects of antioxidants on ultraviolet radiation-indued DNA damage

摘要

摘要:
目的探讨抗氧化剂-丁羟甲苯、维生素C和维生素E是否对紫外线照射诱发的DNA损伤具有拮抗作用.
方法提取人外周血中的淋巴细胞, 用单细胞凝胶电泳法检测经不同浓度丁羟甲苯、维生素C和维生素E处理后并用紫外线照射所诱发的淋巴细胞DNA损伤状况.
结果随着紫外线照射计量的增加, 淋巴细胞的DNA损伤程度逐渐加重; 外源性丁羟甲苯、维生素C和维生素E的加入可明显减轻DNA损伤程度, 丁羟甲苯和维生素C的拮抗作用与其浓度有关, 维生素E的拮抗作用则具有剂量依赖性.
结论丁羟甲苯、维生素C和维生素E对紫外线照射诱发的DNA损伤均有拮抗作用, 但丁羟甲苯和维生素C的作用具有一定的双重性, 维生素E是拮抗紫外线照射诱发DNA损伤较为理想的拮抗剂.

Abstract:
Objective To investigate the role of antioxidants-butylated hydroxytoluene, vitamin C and vtiamin E in UVR-induced DNA damage.
Methods After extracting human peripherial blood lymphocytes and treating with butylated hydroxytoluene, vitamin C and vitamin E as well as ultraviolet radiation, the damaged degree of lymphocytic DNA was ex amined by single cell gel electrophoresis.
Results With the UVR dose increasing, the damaged degree of lymphocytic DNA increase gradully; after treating with three antioxidants UVR-induced DNA damage was reduced, the effects butylated hydroxytoluene and vitamin C relate to its concentration and vitamin E showed dose-dependent manner.
Conclusion Butylated hydroxytoluene, vitamin C and vitamin E possessed antagonism to UVR-induced DNA damage but butylated hydroxytoluene and vitamin C possessed dual nature, vitamin E was a sort of perfect antioxidants in antagonism to UVR-induced DNA damage.

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