婚检人群乙、丙型肝炎、梅毒及艾滋病毒检测

何忠平; 庄辉; 董庆鸣; 李朋; 朱林

婚检人群乙、丙型肝炎、梅毒及艾滋病毒检测

Determination on HBV, HCV, syphilis and HIV infection in population of health checkup before marriage

摘要

摘要:
目的了解北京市婚检人群乙型和丙型肝炎、梅毒及艾滋病病毒感染情况。
方法乙型肝炎表面抗原、丙型肝炎抗体和艾滋病毒抗体采用酶免法检测; 梅毒采用快速血浆反应素环状卡片试验(RPR)和梅毒螺旋体血凝试验(TPHA)检测: 乙型和丙型肝炎病毒载量采用PCR荧光定量方法检测, 病毒基因型采用直接PCR基因分型法测定。
结果 800例婚检人群乙型和丙型肝炎感染率分别为12.0%, 60%, 梅毒和艾滋病病毒未检出。96例乙肝表面抗原阳性婚检人群中, 乙型肝炎病毒载量小于10⁵拷贝/ml者占88.5%, 大于10⁵拷贝/ml者仅占11.5%。5例丙型肝炎病毒RNA阳性者中, 病毒载量均小于10⁴拷贝/ml。该婚检人群HBV感染者中, B型占18.6%, B和C混合型占42.4%, C型占23.7%。3例丙型肝炎病毒感染者均为HCV1b型。
结论该婚检人群中, 乙型和丙型肝炎病毒感染率相对较高, 但病毒载量相对较低, 未发现梅毒和艾滋病毒感染者.

Abstract:
Objective To study HBV, HCV, syphilis and HIV infections in the population of health checkup before marriage in Beijing.
Methods HBsAg, anti-HCV and anti-HIV were detected by enzyme-linked immunoso rbent assays (ELISAs), syphilis was tested by a rapid plasma regain assay(RPR)and a T reponema pallidum hemagg lutination assay (TPHA).Viral load of HBV and HCV were measured by fluorescence quantitative poly merase chain reactions(FQ-PCR), and HBV and HCV genotypes were determined by PCR using type-specific primers.
Results In 800 individuals of health checkup before marriage in Beijing, the prevalence rates of HBV, HCV infections were 12.0% and 6.0%, respectively.No syphilis and HIV infections were detected.In 96 HBsAg positive individuals, 88.5% had the HBV loads less than 10⁵ copies/ml, and 11.5% had more than 10⁵ copies/ml.18.6% were infected with HBVB genotype alone, 42.4% coinfected with B and C genotypes, and 23.7% with genotype C alone.3 HCV individuals were all infected with genoty pe1b.
Conclusion The HBV and HCV prevalence rates were relatively higher, and no syphilis and HIV infections were detected in the population of health checkup before marriage in Beijing.

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