高级检索
龙军, 陈清, 俞守义. 多重PCR检测金葡菌肠毒素基因型的研究[J]. 中国公共卫生, 2003, 19(12): 1427-1428.
引用本文: 龙军, 陈清, 俞守义. 多重PCR检测金葡菌肠毒素基因型的研究[J]. 中国公共卫生, 2003, 19(12): 1427-1428.
shu
LONG Jun, CHEN Qing, YU Shou-yi. Multiplex PCR for identification of staphylococcus aureus enterotoxin gene types[J]. Chinese Journal of Public Health, 2003, 19(12): 1427-1428.
Citation: LONG Jun, CHEN Qing, YU Shou-yi. Multiplex PCR for identification of staphylococcus aureus enterotoxin gene types[J]. Chinese Journal of Public Health, 2003, 19(12): 1427-1428.
shu

多重PCR检测金葡菌肠毒素基因型的研究

Multiplex PCR for identification of staphylococcus aureus enterotoxin gene types

    摘要
  • 摘要:
      目的   应用多重聚合酶反应(PCR), 建立检测金黄色葡萄球菌肠毒素(SE)基因方法。
      方法   用溶菌素和蛋白酶K制备模板DNA, 用多重聚合酶链反应扩增的方法, 对临床分离的金黄色葡萄球菌130株进行扩增。
      结果   金黄色葡萄球菌肠毒素血清型A(SEA)阳性的占23.1%(30/130), 血清型B(SEB)阳性的占43.1%(56/130), 血清型C(SEC)阳性的占11.6%(15/130), 血清型D(SED)阳性的占6.12%(7/130), 血清型EA(SEE)阳性的占2.31%(3/130);femA基因片段在多重PCR中作为内部参照避免了假阴性结果的出现。
      结论   多重PCR技术检测金黄色葡萄球菌肠毒素基因型, 具有敏感、快速、特异的特点, 是一种可靠的实验诊断手段

     

    Abstract:
      Objective   To establish a sensitive and speedy multiple po lymerse chain reaction(multiplex PCR)technique for staphyloccus aureusentero to xin gene types.
      Methods   Template DNA of bacteria was prepared by lysostaphine and Proteinase K.130 strains staphylococcus aureus were amplified by multiplex PCR strategy.
      Results   The SEA gene positive rate of 130 staphylococcus aureus was 23.1%(30/130).The SEB, SEC, SED, SEE gene positive rates of 130 staphylococcus aureus were 43.1%(56/130), 11.6%(15/130), 6.12%(7/130)and 2.31%(3/130)respectively.The femA gene was used as a PCR internal control to avoid the presence of fals-enegative results.
      Conclusion   The multiplex polymerase chain reaction was a specific sensitive and speedy method for the detection of staphylococcus air eusenterotox in genes.

     

    • HTML全文
    • 参考文献(9)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回