大豆异黄酮对成骨细胞生长因子表达的影响
Effect of soybean isoflavone on ICF-1 expression of osteoblstic cells
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摘要:目的 探讨大豆异黄酮对成骨细胞胰岛素样生长因子-1(IGF-1)表达的影响。方法 体外培养新生大鼠颅骨成骨细胞, 然后用塞唑盐(MTT)法测定大豆异黄酮对成骨细胞的增殖作用, 过氧化物酶标记的链霉卵白素(SP)免疫组织化学染色试剂盒检测IGF-1抗体表达, 用原位杂交方法检测IGF-1mRNA基因表达, 用HPIAS-1000图象分析仪对各组表达进行定量分析。结果 大豆异黄酮培养的大鼠第3代成骨细胞的IGF-1抗体和IGF-1mRNA呈阳性表达, 并显著高于阴性对照组(P < 0.05), 阳性细胞胞浆呈棕黄色阳性表达。结论 大豆异黄酮可通过促进大鼠成骨细胞IGF-1分泌而影响成骨细胞形成。Abstract:Objective To study the effect of soybean isoflavone on IGF-1 expression of osteoblastic cells.Methods The osteoblstic cells from cranial bone of new born rat was cultured.The effect of soybean isoflavone on osteoblastic cells proliferation was measured by MTT.The Streptavidin-Peroxidase Histostain TM-Plus Kits was used to detect IGF-1 expression.ICF-1mRNA was detected with situ hybridization.The quantitative analysis was monitored with HP IAS-1000 photograph analysis meter.Results The IGF-1 and IGF-1 mRNA expression of the third generation osteoblastic cells cultured with soybean isoflavone was positive expression and was higher signifiantly than negative control group(P < 0.05).The plasm of positive cells was brown and yellow expression.Conclusion The soybean isoflvone may affect the formation of osteoblastic cells through promoting the secretion of IGF-1.
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