番茄糊对亚硝胺攻击的大鼠抗氧化的影响
Effects of tomato paste on antioxidant in rats induced by nitrosamine
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摘要:目的 研究番茄糊对甲基戊基亚硝胺(MANA)攻击的大鼠体内抗氧化状态的影响。方法 选用Wistar大鼠, 分为3组: MANA组(M)、MANA+番茄糊组(M+TP)及对照组(C)。实验期为26周, 检测血清活性氧单位(ROSunit、超氧化物歧化酶(SOD)、丙二醛(MDA)以及全血谷胱甘肽过氧化物酶(GSH-Px)水平, 同时观察各脏器病理变化。结果 3组大鼠体内随着实验进程抗氧化能力升高及氧化产物降低, 之后, 抗氧化能力下降和氧化产物升高。与M组相比, 实验第22周后, M+TP组大鼠血中ROS单位、SOD、GSH-Px活性显著增高(P < 0.01), MDA含量显著降低(P < 0.01)。M组和M+TP组大鼠食管和胃均发生癌前病变及癌变, 而M+TP组第22周大鼠食管非典型增生及26周癌变发生率低于M组。结论 大鼠在接受MANA刺激之后体内氧化、抗氧化状态呈现先增高后降低的动态改变, 通过添加番茄糊可以提高大鼠抗氧化能力, 推测与抑制亚硝胺的致癌性有关Abstract:Objective To study effects of tomato paste on antiox idation in rats induced by N-methy l-N-amylni-trosamine(MANA).Methods Wistar rats were randomly divided into three groups: simple MANA(M), MANA plus to matopaste(M+TP)and contro l(C)group.The experiment lasted for 26 weeks.The levels of ROS unit, SOD, GSH-Px and MDA in the blood were determined and histolo gical examination was done.Results The levels of antiox idant in the blood of rats were decreased and content of oxidation were increased as the experiment lasted.Compared with M group, activity of ROS unit, SOD.GSH-Px in M+TP group were significantly higher and content of MDA in M+TP was significantly lower than those in M group(P < 0.01)after 22 weeks.Precancerous lesions and carcinoma of esophagus or forestomach were observed in M and M+TP groups.The incidence of typical hyperplasia in week 22 and carcinoma in week 26 in M+TP group were lower than those in M group.Conclusion The levels of antioxidant and oxidant in the blood of rats were dynamic raised first and decreased later.The inhibitory effect may be due to tomato paste which may improve antioxidation in rats.
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