高级检索
王洪军, 王继群, 周莉, 王桂君, 金宁一, 殷震. Gag/IFN α-2b融合基因在痘苗病毒中共表达研究[J]. 中国公共卫生, 2003, 19(12): 1447-1449.
引用本文: 王洪军, 王继群, 周莉, 王桂君, 金宁一, 殷震. Gag/IFN α-2b融合基因在痘苗病毒中共表达研究[J]. 中国公共卫生, 2003, 19(12): 1447-1449.
shu
WANG Hong-jun, WANG Ji-qun, ZHOU Li, . Study on coexpression of HIV-1 gag/IFN α-2b fusion gene in vaccina virus[J]. Chinese Journal of Public Health, 2003, 19(12): 1447-1449.
Citation: WANG Hong-jun, WANG Ji-qun, ZHOU Li, . Study on coexpression of HIV-1 gag/IFN α-2b fusion gene in vaccina virus[J]. Chinese Journal of Public Health, 2003, 19(12): 1447-1449.
shu

Gag/IFN α-2b融合基因在痘苗病毒中共表达研究

Study on coexpression of HIV-1 gag/IFN α-2b fusion gene in vaccina virus

    摘要
  • 摘要:
      目的   本实验将编码AIDS病毒(HIV-1)核心蛋白gag与编码干扰素(IFN α-2b)基因插入以痘苗病毒复制非必需区血凝素(HA)基因为侧翼, 牛痘病毒包涵体(ATI)启动子和串联的p75突变型早期启动子为基本构件的痘苗病毒表达载体(pJ38), 经与野生型痘苗病毒在细胞内同源重组, 获得具有生物活性的重组痘苗病毒vJ38gag/IFN α-2b。
      方法   经免疫荧光、Dot-ELISA、SDS-PAGE、Western-blot等方法检测表达产物, 分析免疫小鼠后血清抗体IgGOD490值与T淋巴细胞的变化。
      结果   vJ38gag/IFN α-2b能表达Gag/IFN α-2b融合蛋白, 分子量约60kDa。免疫小鼠实验表明血清抗体IgGOD490值、CD4+、CD8+T淋巴细胞计数的组间比较(P < 0.001), 含IFN α-2b与单纯vJ38gag组相比(P > 0.05), 但呈渐进性增高的趋势。
      结论   重组痘苗病毒表达的gag/IFN α-2b融合蛋白具有良好的免疫原性和免疫反应性

     

    Abstract:
      Objective   pJ38 was used as expression vector, gag gene encoding type iv core protein of AIDS virus(HIV) and encoding interferon A-2b gene of human were inserted into the downstream of the hybrid promotor, where HA gene of the vaccinia virus unnecessary areas was the flank and there were 38p7.5 promotor of mutant-type vaccinia virus, A-type inclusion late promotor of vaccinia virus.AvJ38gag/IFN α-2b was acquired by homologous recombination with the natural vaccinia virus as selection of the HA-plaque for reporter.
      Methods   The IFA, Dot-blot, SDS-PAGE, Western-blot and FCM were used.
      Results   The recombinant viruses could express gag/IFN α-2b, whose molecular weight was 60 kDa.In the mice immunization experiment, the OD490 value of the serum antibodies of HIV-1 was different(P < 0.001)in groups, There was not difference between containing IFN α-2b and vJ38Gag.
      Conclusion   Gag/IFN α-2b proteins both had good reactinogenicity and immunogenictiy.

     

    • HTML全文
    • 参考文献(6)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回