Abstract:
Objective To explore the effect of lipid peroxidation on injury of testis induced by electromagnetic pulse (EMP)irradiation in mice.
Methods 114 male Kunming mice were randomly divided into irradiated group and control groups, content of malondialdehyde(MDA)and activity of superoxide dismutase(SOD)in testis were measured, and pathological changes of testis were observed at 6 h, 1, 3, 7, 14 and 28 d after miceirradiated gener ally by 8×103, 2×104 and 6×104 v/m EMP respectively for five times.
Results There was a opposite tendency between alteration of SOD activity and change of MDA content in testicular tissue after EMP irr adiation, alteration of SOD activity happened quickly, SOD activity lowered siginificantly at 6 h and 14 d after 8×103 v/m EMP irradiation, from 1 to 14 d after 2×104 v/m EMP irradiatton, there was obvious increaseprior to obvious decrease after 6×104 v/m EMP irradiat ion, but MDA contentheightened significantly at 14 d, which lagged behind obviously alteration of SOD activity.It showed EMP irradiation also caused striking edema, necrosis and shedding of spermatogenic cells, scarceness and deletion of mature sperm within seminiferous tubule, edema, congestion and hemorrhage within interstitial.
Conclusion Decrease of SOD activity, potentiation of lipid peroxidation took part in occurrence and prog ress process of spermatogenic cell injury induced by electromagnetic pulse irradiation.
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