结核杆菌感染对人肺组织β-防御素的作用

Effect of mycobacterium infection on β-defensin in human lung tissue

摘要:
目的探讨结核杆菌对人肺组织β-防御素-1, 2基因的表达调控。
方法取肺结核病人病变部分的肺组织, 提取RNA, 采用RT-PCR技术扩增人β-防御素hBD-1cDNA, hBD-2cDNA, 并重组到pGEM-TEasy载体, 经限制性内切酶谱分析和DNA序列测定证实。
结果所克隆的cDNA片段分别为hBD-1, hBD-2的cDNA序列; 肺结核病人肺组织hBD-诱导表达, hBD-1固有表达增强。
结论结核杆菌感染可正相调节人肺组织hBD-1, hBD-2的表达

Abstract:
Objective To study the regulation of human β-defensin-1, 2 gene expression by mycobacterium infection.
Methods RNA was isolated from mycobacterium-infected human lung tissue.The human β-defensin-1, 2 cDNA fragment was obtained by RT-PCR amplification.The RT-PCR products around 300 bp, 270 bp in size were cloned separately into pGEM-T easy vector and the recombinant clones were analyzed by digestion with restriction endonucleases and DNA sequencing.
Results Sequence analysis confirmed that the RT-PCR products were hBD-1 cDNA and hBD-2 cDNA respectively.The expression of hBD-2 gene was induced in mycobacterium-infected human lung tissue, while the expression of hBD-lgene was enhanced.
Conclusion Mycobacterium infection could up-regulate hBD-1, 2 gene expression in human lung tissues.