山东省HIV-1B亚型毒株分子流行病学研究

Molecular epidemiology analysis on HIV-1 subtype B strains found in Shandong province

摘要:
目的了解人类免疫缺陷病毒1型(HIV-1B)亚型毒株在山东省的流行分布情况和传播规律。
方法采集17份HIV-1感染者的外周静脉防凝血, 提取前病毒DNA进行体外扩增, 获得包膜蛋白(env)基因的核酸片段, 并对其C2-V3及邻区的核苷酸进行测定和分析。
结果系统树和基因离散率分析发现这17份血样均为HIV-1B亚型感染, 且感染毒株距离泰国B亚型代表株RL42最近, 离散率为9.16±3.34, 17毒株之间的离散率为11.69±4.19。氨基酸序列分析发现, V3环顶端4肽基序呈多态性, 有6种形式。
结论 HIV-1B亚型在山东省地理分布广, 并涉及3类人群两种传播途径, 且毒株出现较大变异, 具有广泛传播的潜在危险

Abstract:
Objective To investigate the prevalent status of HIV-1 subtype B epidemic in Shandong province.
Methods 17 DNA fragments of HIV-1 envgene were amplified by nested PCR from persons infected with HIV-1.Their C2-V3 and adjacent region were sequenced.
Results These 17 cases were confirmed to be infected with HIV-1 subetype B strain (sd-B), the genetic distance between sd-B and Thailand subetype B strains was nearest, 9.16±3.34, the innergr oups distance of sd-B was 11.69±4.19.The motif of top 4-peptides of V3 loop showed 6 forms.
Conclusion Subtype B strain generated obvious variation, and it distributed extensively in population and district in Shandong province.