火棘对D-半乳糖小鼠抗氧化作用的影响

侯建军; 魏文科; 黄惠; 武模戈

火棘对D-半乳糖小鼠抗氧化作用的影响

Antioxidation effects of pyracantha on aging mice model induced by overdose of D-galactose

摘要

摘要:
目的研究火棘对D-半乳糖所致衰老小鼠的抗氧化作用。
方法将小鼠随机分为4组, 分别喂以标准饲料(空白对照组和D-半乳糖对照组)及标准饲料中添加15%和30%的火棘果实粉(分D-半乳糖火棘组Ⅰ、Ⅱ2组), 同时各D-半乳糖组小鼠每天颈背皮下注射D-半乳糖80mg/kg, 连续给药造模42d后, 根据试剂盒说明分别检测各相关抗氧化指标(MDA, SOD, GSH-Px, GSH)。
结果 D-半乳糖火棘组Ⅰ、Ⅱ两组小鼠心、肝、脑的MDA含量降低, 红细胞、肝、脑SOD的活性升高, 全血、肝GSH-Px活性和GSH含量升高, 胸腺指数回升。
结论火棘有一定的抗氧化作用, 并能延缓由D-半乳糖所致衰老小鼠的衰老过程。

Abstract:
Objective To study the anti-aging of pyracantha on aging mice model induced with overdose of D-galactose.
Methods Four groups of mice were fed respectively with standard diet(2 control groups), 85% standard diet plus 15% pyracantha (group Ⅰ), and 70% standard diet plus 30% pyracantha(group Ⅱ), while the aging model was induced by subaxillary injection of D-galactose 80 mg/kg for 42 days, the MDA, SOD, GSH-Px, GSH were determinated according to the technical manual of reagent.
Results At the end of the experiment, the contents of Malondialehyde(MDA)in liver, heart and brain of groupⅠandⅡdeceased significantly contrast to D-galactose control group, and the activities of superoxide dismatase(SOD)in red blood cell, liver and brain increased, the activities of glutathione peroxidase(GSH-Px)and the contents of glutathione(GSH)increased in blood and liver, and the thymus weight index increased, too.
Conclusion It was suggested that Pyracanthy had some antioxidation and might prolong the aging process of mice model induced by overdose of D-galactose.

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