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王学习, 朱玉真. 硫酸镍对雌性大鼠性腺机能的影响[J]. 中国公共卫生, 2003, 19(8): 946-947.
引用本文: 王学习, 朱玉真. 硫酸镍对雌性大鼠性腺机能的影响[J]. 中国公共卫生, 2003, 19(8): 946-947.
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WANG Xue-xi, ZHU Yu-zhen. Effect of nickel sulfate on gonad of female rats[J]. Chinese Journal of Public Health, 2003, 19(8): 946-947.
Citation: WANG Xue-xi, ZHU Yu-zhen. Effect of nickel sulfate on gonad of female rats[J]. Chinese Journal of Public Health, 2003, 19(8): 946-947.
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硫酸镍对雌性大鼠性腺机能的影响

Effect of nickel sulfate on gonad of female rats

    摘要
  • 摘要:
      目的   观察硫酸镍(NiSO4)腹腔注射染毒对雌性大鼠下丘脑-垂体-性腺轴的毒作用及其机制。
      方法   对健康性成熟Wistar雌性大鼠腹腔注射NiSO4 5.00, 2.50, 1.25mg/kg染毒, 0.2ml/100g体重, 1次/d, 连续21d。阴道脱落上皮细胞涂片10d观察动情周期, 第22d皮下注射人工合成促性腺激素九肽类似物(GnRH)前放免法测血清卵泡刺激素(FSH)、黄体生成素(LH)、孕酮(P)、雌二醇(E2), 注射GnRH后放免法测血清FSH、LH。第24d腹腔注射孕马血清促性腺激素(PMSO), 48h后腹腔注射人绒毛膜促性腺激素(HCG)后体视显微镜下挤出卵丘团, 计数每只动物的超排卵数。同时取阴道、子宫、卵巢、垂体测镍含量。
      结果   5.00, 2.50mg/kg组动物阴道、子宫、卵巢与NS组比较镍含量有显著性差异; 1.5mg/kg组动物子宫、卵巢镍含量与NS组比较有显著性差异; 染毒组的动物出现动情周期延长; 注射GnRH前, 5.00mg/kg组血清FSH、LH与NS组比较有显著性差异, 注射GnRH后各染毒组血清FSH、LH与NS组比较均无显著性差异; 染毒5.00, 2.50mg/kg组血清E2、P含量与NS组比较有显著性差异; 5.00mg/kg组动物超排卵数与NS组比较有显著性差异。
      结论   腹腔注射硫酸镍染毒可引起雌性大鼠卵巢损伤, 其机制可能与镍的直接作用。

     

    Abstract:
      Objective   To study the toxic effects of nickel sulfate on hypothalamic-pituitary-ovarian axis of female rats.
      Methods   Wisth female rats were given by abdomen with different doses of nickel sulfate(5.00, 2.50, 1.25 mg/kg)for 21 days and the estrus cycle of rats was observed, the changes of Ni levels in vagina, uterus, ovary and hypophysis were measured, respectively.The GnRH-stimulating test were carried in rats, with the serum FSH, LH, P and E2 determined before the injection of GnRH and the serum FSH, LH determined after the injection of GnRH finally the super-ovulation test was done.
      Results   The Ni contents in the vagina, uterus and ovary of the 5.00, 2.50mg/kg rats were higher than in the control(P < 0.01).The Ni contents in the uterus and ovary of the 1.25mg/kg rats were higher than in the control(P < 0.05);before the injection of GnRH, the levels of FSH and LH were higher than in the control(P < 0.01), but there were no significant difference of serum FSH and LH between groups after the injection of GnRH; The levels of serum E2, P of the 5.00 2.50 mg/kg rats were lower than in the control(P < 0.05 orP < 0.01); There was significant difference of the amoount of ova between the 5.00mg/kg group and the control group in the super-ovulation test.
      Conclusion   The ovary of the female rats were damaged by nickel sulfate given by abdomen.

     

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