抗HBV药物对小鼠早期胚胎发育的影响
Effect of lamivudine and interferon-α on development of early mouse embryos in vitro
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摘要:目的 观察拉米夫定(3TC)和干扰素(IFNα)2种抗乙型肝炎病毒(HBV)药物对早期胚胎发育的影响, 探讨妊娠早期用药的可能性。方法 收集昆明种小鼠的单细胞受精卵, 采用微滴培养法连续培养72h, 观察不同浓度3TC和IFNα对胚胎发育的影响, 以胚胎发育到各期的比例为判断发育效果的标准。结果 当IFNα浓度高达1000u/m; 3TC500μmol/L时, 胚胎发育率呈下降趋势, 尤其是培养液中3TC浓度达500μmol/L时, 发育至4-细胞、8-细胞及桑椹胚期的胚胎数明显减少(P < 0.05), 但在IFNα浓度 < 500U/ml; 3TC < 300μmol/L时, 对小鼠早期胚胎发育基本无抑制作用。结论 在正常治疗剂量下, IFNα和3TC对早期胚胎发育无影响。Abstract:Objective To study the effects of lamivudine(3TC)and interferon-(IFN)on development of early embryos in vitro and to investigate the possibilty of antiviral therapy during first-trimester pregnancy.Methods One-cell mouse embryos collected from the oviduct of the superovulated mice were cultured in microdrops of medium for 72 hours, the effects of 3TC and IFN were observed in various concentration on development of embryos, and the percentage of embryos developing to various stages of embryos was used as evaluation criteria.Results The development rate of embryos was decreaed significantly in the concentration of 1 000U/ml(IFN)and 500 mol/L(3TC), two anti-HBV drugs had no inhibitive effect on development of early mice embryos under the concentrations of 500U/ml(IFN)and 300 mol/L(3TC).Conclusion IFN and 3TC had no inhibitive effect on development of early embryos at the proper therapeutic concentration.
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