二氧化硫对大鼠肺细胞膜通透性的损伤效应

杜青平; 孟紫强

二氧化硫对大鼠肺细胞膜通透性的损伤效应

Damage effects of sulfur dioxide on pulmonary membranous permeability of rats

摘要

摘要:
目的探讨二氧化硫(SO₂)吸入对大鼠肺组织及肺细胞通透性的影响。
方法用生理盐水灌洗大鼠支气管肺泡, 收集肺泡灌洗液(BALF)和肺泡巨噬细胞(AM), 从整体、细胞和亚细胞水平研究SO₂对肺组织和细胞的通透性的影响。
结果 SO₂吸入后, 大鼠体重增加减慢, 肺组织湿重增大, 干重减小; 肺泡BALF中蛋白质含量和肺通透指数增加; 肺血管通透性增大; BALF中乳酸脱氢酶(LDH)和酸性磷酸酶(ACPase)活力升高, 而AMLDH和ACPase活力降低。
结论 SO₂导致大鼠肺组织损伤, 肺血管通透性增加, AM膜通透性增加, 细胞器(如溶酶体)结构和功能发生改变。

Abstract:
Objective To study the effects and mechanisms of SO₂ on lungs and the cell membrane permeability of pulmonary cells.
Methods The brochoalveolar lavage fluid(BALF)and the pulmonary cells were gathered to study the effects and mechanisms of SO₂ on lungs and the membrane permeability of pulmonary cells on the body, cellular and subcellular levels.
Results The increase of body weight and the lung dry weight decreased, otherwise the lung wet weight increased; The protein contents in the BALF, the pumonary vascular permeability index and alveolar permeability increased as well as the lactic dehydrogenase(LDH)and the acid phophatase(ACPase)activity in BALF increased.The LDH and ACPase activity in AM decreased.
Conclusion The pulmonary tissues were damaged, the pulmonary vascular permeability and the membranous permeability of the cells increased, and the structure and function of the organelles(e.g lysosome)were changed.

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