色氨酸对疟原虫配子体激活因子的影响

Effect of tryptophan on malaria gametocyte activating factor in salivary glands of anopheles stephensi

摘要:
目的探讨色氨酸代谢产物对斯氏按蚊唾液腺抽提物中疟原虫配子体激活因子的影响。
方法应用柏氏疟原虫体外雄配子体出丝分析方法检测果糖喂饲液中掺入色氨酸后斯氏按蚊唾液腺抽提物疟原虫配子体激活因子的活性变化。
结果斯氏按蚊唾液腺中柏氏疟原虫配子体激活因子与按蚊生长发育程度呈同步变化; 喂饲液中掺入色氨酸可增强按蚊唾液腺的出丝诱导活性。
结论喂饲液中掺入色氨酸可提高斯氏按蚊唾液腺中疟原虫配子体激活因子的含量。

Abstract:
Objective To investigate the effect of tryptophan metabolite in the salivary glands of anopheles stephensi on malaria gametocyte activating factor.
Methods The change of activity of malaria gametocyte activating factor in the salivary glands of anopheles stephensi fed on fruoctose supplemented with tryptophan by exflagellation assay of plasmodium berghei in vitro.
Results The content of gametocyte activating factor in the salivary gland of anop heles stephensi was related with growth and development of the mosquitoes; exflagellation-inducing activity in the salivary gland was increased by supplementing the diet of mosquito with tryptophan.
Conclusion The addition of tryptophan in the diet of mosquito increased the content of malaria gametocyte activating factor in the salivary gland of anopheles stephensi.