反相高效液相测定洛伐他汀方法的改良

谢虹; 邱相君; 付琳; 王桂珍

反相高效液相测定洛伐他汀方法的改良

Improvement of RT-HPLC on determination of lovastatin

摘要

摘要:
目的探索采用反相高效液相色谱法(HPLC), 提高检出血浆洛伐他汀浓度及缩短测定时间。
方法 HPLC条件: 色谱柱YWG-C₁₈(10μm); 流动相: 甲醇: 水(83:17, V/V); 柱温25℃; 检测波长237nm。血浆经环己烷和异丙醇萃取浓缩后, 直接进样测定洛伐他汀的浓度。
结果血浆洛伐他汀浓度在2.0~80.0μg/L范围内线性关系良好, 回归方程式y=241.56+197.25x(r=0.9991); 最低检测限为20μg/L; 平均回收率为(96.01±7.17)%。测定一个样品的时间只需6min。
结论血浆前处理充分, 流动相甲醇与水比例适当是提高检测限和缩短测定样品时间的关键, 有一定的使用价值。

Abstract:
Objective To improve the detection concentration and time of plasma lovastatin by studying the RP-HPLC method.
Methods The condition of HPLC: The analytical column was packed with YWG-C₁₈(10μm).The mobile phase was methanol-water(83:17, V/V).The temperature of column was 25℃.The compounds were quantified using a ultraviolet detector operated at 237 nm.The lovastatin in plasma sample extracted by cycllohexane-isopropyl alcohol.
Results The liner range of plasma lovastatin was 2.0-80 ng/ml.The regression recation was y=241.56+197.25x(r=0.999 1).The limit of detection was 2.0μg/L and the mean recovery was(96.01±7.17)%.The determination of a sample only needed 6 min.
Conclusion Controlling the pre2 treatment of plasma and the ratio of methanol to water was the way to enhance the detection limit and shorten detection time.

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