大气污染对大鼠细胞因子的影响
Effect of air pollution on IL-4, IFN-r in serum of rats
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摘要:目的 分析大气污染对大鼠细胞因子的影响, 以揭示大气污染对呼吸系统损害及机体影响的机制, 为预防和治疗大气污染对健康的损害提供依据。方法 将体重在180~220g的大鼠随机分为4组, 即对照组、低剂量组、中剂量组、高剂量组, 分别进行颗粒物和SO2、NO2染毒, 颗粒物染毒采用气管注入法, 各注入颗粒物75, 15, 30mg, 灌注体积为1ml, 对照组注入同体积的生理盐水。染尘后的第2d, 再进行SO2和NO2混合气体染毒, 低、中、高剂量组的SO2和NO2的浓度分别为8, 5mg/m3; 16, 10mg/m3; 32, 20mg/m3, 对照组为空气。连续染毒7d后, 于染毒后的第1d和第8d取腹主动脉血, 采用ELISA法测定血清中IL-4和IFN-r。结果 染毒后第1d, 中、高剂量组大鼠血清中IL-4显著高于对照组(P < 0.05), 到染毒后第8d, 中剂量组IL-4水平与对照组无差异, 但高剂量组仍显著高于对照组。而大鼠血清IFN-r呈下降趋势, 中、高剂量组大鼠血清IFN-r显著低于对照组, 到染毒后第8d, 仍未恢复正常。升高的IL-4促进IgE分泌, IFN-r的减少使对IL-4的抑制作用减弱, 也间接使IgE升高。结论 大气污染物可诱导大鼠IL-4的增高和IFN-r的下降, 从而导致TH1与TH2之间失衡, 使呼吸系统疾病和变态反应性疾病发生。Abstract:Objective To analyse the effect of air pollution on cytokine in rats and explore the damage mechanism of air pollution to respiratory system.Methods Rats weighting 180-220 g were randomly divided into 4 groups, which were control group, lower, middle and higher dose groups.Rats of dose groups were separately poured into particles 715, 15 and 30 mg by upper respiratory tract and exposed to SO2, NO2 8, 5 mg/m3; 16, 10 mg/m3; 32, 20 mg/m3 for seven days.On the first day and the eighth day after poisoned, common abdomen artery blood were collected and IL-4, IFN-r were measured by ELISA.Results On the first day after exposed pollutants, IL-4 in middle, higher dose group rats were significantly higher than that of control rats(P < 0105).On the eighth day, IL-4 fall down again, but still higher than that of control rats.IFN-r in serum showed decrease on the first day after exposed to pollutants and still was not recovery on the eighth day.Conclusion Air pollutants could induce IL-4 increasing and IFN-r decreasing, which damage the equilibrium between TH1 and TH2, and cause respiratory disease and allergy disease.
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