斑须按蚊产卵对不同器官出丝诱导物质的影响

王继春; 曹雅明; 罗恩杰

斑须按蚊产卵对不同器官出丝诱导物质的影响

Effects of oviposition of anopheles stephensi on malaria exflagellation inducing substance in the different organs

摘要

摘要:
目的探讨雌性斑须按蚊产卵对蚊体内不同器官中疟原虫雄配子体出丝诱导物质的影响。
方法应用体外雄配子体出丝分析方法, 检测吸血后产卵的雌性斑须按蚊头、唾液腺和卵巢组织中出丝诱导物质对柏氏疟原虫雄配子体出丝诱导活性的变化。
结果斑须按蚊头匀浆上清中所含出丝诱导物质的活性与产卵是否完成没有显著关系; 按蚊产卵完成后, 唾液腺抽提物的出丝诱导活性显著升高, 而卵巢匀浆上清的诱导活性显著降低。
结论雌性斑须按蚊产卵影响唾液腺雄配子体出丝诱导物质的活性。

Abstract:
Objective To investigate the effect of the female anopheles stephensi mosquitoes.oviposition on the malaria exflagellation-inducing substance in the different organ of the mosquitoes.
Methods The change of exflagellation-inducing activity in the heads, salivary glands and ovaries of anopheles stephensi was analyzed by using vitro exflagellation assay of plasmodium berghei.
Results There was no apparent relationship between the exflagellation-inducing activity of the head supernatant and completely egg-laid or incompletely egg-laid mosquitoes.After complete oviposition of eggs, the exflagellation-inducing activity in the salivary gland extract significantly increased and the activity in the ovary super natant significantly reduced.
Conclusion The female anopheles stephensi mosquitoes' laying egg exerted its effect on the exflagellationinducing activity in the salivary glands.

HTML全文

参考文献(10)

施引文献

资源附件(0)