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李贵昌, 吴坤, 赵艳, 赵岚. 维生素E琥珀酸酯诱导胃癌细胞凋亡[J]. 中国公共卫生, 2003, 19(6): 688-689.
引用本文: 李贵昌, 吴坤, 赵艳, 赵岚. 维生素E琥珀酸酯诱导胃癌细胞凋亡[J]. 中国公共卫生, 2003, 19(6): 688-689.
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LI Gui-chang, WU Kun, ZHAO Yan, . Apoptosis induced by vitamin E succinate in gastric cancer cells[J]. Chinese Journal of Public Health, 2003, 19(6): 688-689.
Citation: LI Gui-chang, WU Kun, ZHAO Yan, . Apoptosis induced by vitamin E succinate in gastric cancer cells[J]. Chinese Journal of Public Health, 2003, 19(6): 688-689.
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维生素E琥珀酸酯诱导胃癌细胞凋亡

Apoptosis induced by vitamin E succinate in gastric cancer cells

    摘要
  • 摘要:
      目的   探讨维生素E琥珀酸酯(VES)诱导人胃癌SGC-7901细胞凋亡执行阶段的机制。
      方法   分别将对照组01%无水乙醇和5, 10, 20μg/mlVES加入培养液中, 24h后通过荧光染色观察细胞核形态和线粒体跨膜电位(ΔΨm)改变, 并用Western蛋白印记检测凋亡诱导因子在线粒体和细胞质中的重新分布。
      结果   VES可明显诱导人胃癌SGC-7901细胞凋亡, 20μg/mlVES可使细胞凋亡率达到497%, 伴有ΔΨm的明显降低, 并有凋亡诱导因子自线粒体中的释放。
      结论   VES可能是通过使线粒体通透性改变, 降低ΔΨm, 并释放线粒体中的凋亡诱导因子, 诱导SGC-7901细胞凋亡。

     

    Abstract:
      Objective   To investigate the molecular mechanism of apoptosis induced by vitamin E succinate(VES)in gastric cancer cell line SGC-7901.
      Methods   Cells were treated with 0.1% anhydrous alcohol as control and VES in 5, 10, 20 μg/ml for 24h.Apoptosis and mitochondrial transmembrane potential(ΔΨm)were morphologcally assessed through staining with fluorochrome.Mitochondria-nuclear translocation of apoptosis inducing factor(AIF)was detected with Western blotting.
      Results   VES can induce apoptosis significantly in gastric cancer cell line SGC-7901.The apoptotic cells were 49.7% when treated with 20 μg/ml VES, which was associated with the decrease of ΔΨ m and the redistribution of AIF from mitochondria to cytoplasm.
      Conclusion   VES may induce apoptosis of SGC-7901 through mitochondrial permeability transition which decreased the ΔΨm and released AIF from mitochondia.

     

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