藻毒素对实验性大鼠肝癌的促进作用

赵金明; 蒋颂辉; 朱惠刚

藻毒素对实验性大鼠肝癌的促进作用

Promotive activity of microcystins during experimental hepatocarcinogenesis in rats

摘要

摘要:
目的研究藻毒素在实验性大鼠肝癌诱导过程中的促进作用。
方法以二乙基亚硝胺(DEN)为启动剂, 藻毒素为促进剂, 构建大鼠肿瘤促进模型(Solt-Farber模型), 观察肝脏病理形态改变, 分别运用免疫组化法和RT-PCR、原位杂交法检测肝细胞中GSTPi蛋白和GSTPimRNA表达情况。
结果实验组动物肝脏产生结节性增生灶, 藻毒素单独作用不能诱导GSTPimRNA及蛋白表达, 但能够促进DEN诱导产生的GSTPimRNA及蛋白表达, 使其表达增加, 并具有一定的剂量-反应关系。
结论藻毒素对实验性大鼠肝癌的发生具有促进作用, 提示具有促癌活性。

Abstract:
Objective To investigate promotive activity of microcystins(MC)during experimental hepatocar cinogenesis in rats, and explore its potential mechanism.
Methods Two-stage hepatocarcino genesis model of rats(Solt-Farber Model) was established with DEN and MC applied as initiator and promoter, respectively.The histophatho logical changes in liver were observed under light microscope, and the expressions of protein and mRNA of GSTPi were detected by means of immunohistochemistry and RT-PCR, ISH, respectively.
Results Hyperplastic and precancerous foci and nodule were induced in liver of rats treated with DEN and MC.Compared to DEN, MCs alone can't induce the expression of GSTPi mRNA and protein, but possess the ability of up-regulating the expression of GSTPi mRNA and protein induced by DEN, and the dose-response relationship was obvious.
Conclusion MCs had potential promotive activity during experimental hepatocarcinogenesis of rat, which indicated it had tumor promotion.

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