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刘志宏, 雷志明, 魏学涛, 薛彬, 徐厚恩. 混合稀土硝酸盐对仔鼠免疫功能的影响[J]. 中国公共卫生, 2003, 19(2): 136-138.
引用本文: 刘志宏, 雷志明, 魏学涛, 薛彬, 徐厚恩. 混合稀土硝酸盐对仔鼠免疫功能的影响[J]. 中国公共卫生, 2003, 19(2): 136-138.
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LIU Zhi-hong, LEI Zhi-ming, WEI Xue-tao, . Effects of RE(NO3)3 on immune function of mice's offspring after gestational exposure[J]. Chinese Journal of Public Health, 2003, 19(2): 136-138.
Citation: LIU Zhi-hong, LEI Zhi-ming, WEI Xue-tao, . Effects of RE(NO3)3 on immune function of mice's offspring after gestational exposure[J]. Chinese Journal of Public Health, 2003, 19(2): 136-138.
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混合稀土硝酸盐对仔鼠免疫功能的影响

Effects of RE(NO3)3 on immune function of mice's offspring after gestational exposure

    摘要
  • 摘要:
      目的   了解混合稀土硝酸盐〔RE(NO3)3〕能否经胎盘进入仔鼠体内及RE(NO3)3对仔鼠免疫功能的影响。
      方法   小鼠妊娠7~8d开始经口灌胃, 分别给予2, 20, 200mg/kgRE(NO3)3, 对照组给与蒸馏水, 直至生下仔鼠.采用电感耦合等离子体质谱法(ICP-MS)检测F1代仔鼠体内稀土元素镧(La)、铈(Ce)、镨(Pr)、钕(Nd)的含量; 分别测定刚离乳及离乳3周仔鼠免疫器官重量、脾抗体形成细胞数(IgM-PFC/脾)、迟发型变态反应(DTH)和巨噬细胞功能。
      结果   妊娠期母鼠经口灌胃RE(NO3)3后, 200mg/kg组F1代仔鼠刚出生时体内稀土元素La高于对照组(P < 0.05);20.0mg/kg组F1代仔鼠刚出生时体内稀土元素La、Ce、Pr、Nd含量均明显高于对照组(P < 0.05).F1代仔鼠刚离乳时, 20.0mg/kg组仔鼠脾脏重量明显高于对照组(P < 0.05);20mg/kgRE(NO3)3可明显增加IgM-PFC/脾(升高82.2%); 2.0, 20.0mg/kg组碳粒廓清指数K值和吞噬指数α值均明显高于对照组(P < 0.01, P < 0.05);20mg/kgRE(NO3)3对DTH有明显升高作用(P < 0.05).F1代仔鼠离乳后3周各剂量组仔鼠脾脏重及其脏体比均明显高于对照组(P < 0.05);200.0mg/kgRE(NO3)3对IgM-PFC/脾有明显的抑制作用(抑制率为3.69%), 且对碳粒廓清指数K值和吞噬指数A也有明显的抑制作用(P < 0.05).各剂量组DTH未见有明显改变。
      结论   RE(NO3)3可经胎盘转运进入仔鼠体内, 且可对仔鼠免疫功能产生一定的影响, 这些影响可能是RE(NO3)3经胎盘转运进入仔鼠体内的结果。

     

    Abstract:
      Objective   To observe the effects of mixed rare earth nitrateRE(NO3)3on immune function of mice off-spring and whether these effects result from RE through the route of placenta.
      Methods   RE(NO3)3 was administered to mice by gavage at dosage of 2, 20, 200 mg/kg during gestational period.The weights of spleen and thy mus, the spleen plaque for ming cells(PFC), the delayed type hypersensitivety(DTH)and the char coal clearance were determined.
      Results   The results after weaning showed that the contents of La, Ce, Pr and Nd in 200mg/kg dosage group were higher than those of control group significantly, at the dosage of 2.0mg/kg, the number of IgM-PFC was increased 82.2%, the rate of clearance and clearance index of 2.0, 20.0mg/kg were higher significantly than that of control(P < 0.05).2.0mg/kg RE(NO3)3 could enhance the DTH significantly(P < 0.05).The results after weaning for 3 weeks sho wed that the number of IgM-PFC of 200mg/kg dose groups were 36.9% lower than that of control group, the rate of clearance and clear ance index of 200mg/kg were lower sig nificantly than that of control(P < 0.05).No significant changes in DTH were observed in each exposed group.
      Conclusion   RE(NO3)3 can affect the immune function of mice.s offspring, which played a role viaplacenta.

     

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