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赵秀兰, 徐贵发, 吴冰. 麦胚黄酮类粗提取物抑制乳腺肿瘤作用机制[J]. 中国公共卫生, 2003, 19(2): 169-171.
引用本文: 赵秀兰, 徐贵发, 吴冰. 麦胚黄酮类粗提取物抑制乳腺肿瘤作用机制[J]. 中国公共卫生, 2003, 19(2): 169-171.
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ZHAO Xiu-lan, XU Gui-fa, WU Bing. Study on inhibition of mammary tumor by flavonoids crude extract in wheat germ[J]. Chinese Journal of Public Health, 2003, 19(2): 169-171.
Citation: ZHAO Xiu-lan, XU Gui-fa, WU Bing. Study on inhibition of mammary tumor by flavonoids crude extract in wheat germ[J]. Chinese Journal of Public Health, 2003, 19(2): 169-171.
shu

麦胚黄酮类粗提取物抑制乳腺肿瘤作用机制

Study on inhibition of mammary tumor by flavonoids crude extract in wheat germ

    摘要
  • 摘要:
      目的   观察麦胚黄酮类粗提取物对DMBA诱导大鼠乳腺肿瘤的抑制作用并探讨其作用机理。
      方法   将70只出生50d的雌性SD大鼠随机分为4组, 分别为: 阴性对照组(10只), 乳腺癌模型组(20只), 低剂量组(20只)和高剂量组(20只)。将15mg7, 12-二甲基苯蒽(DMBA)溶于15ml植物油中, 经灌胃分别给予乳腺癌模型组和实验组大鼠, 阴性对照组给予等量植物油。2周后重复1次。低剂量组和高剂量组动物分别喂饲含2%和10%麦胚黄酮类粗提取物的饲料, 阴性对照组和乳腺癌模型组饲以普通饲料。持续喂养24周。24周后断头处死大鼠, 分别测定血液和肝脏中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、谷胱甘肽硫转移酶(GST)活性及还原型谷胱甘肽(GSH)、丙二醛(MDA)含量。
      结果   实验组大鼠血液和肝脏中的GSH-Px、SOD、GST活性和GSH含量较乳腺癌模型组有明显增加(P < 0.05或P < 0.01);MDA含量则明显下降(P < 0.05)。实验组大鼠乳腺肿瘤的发生率低于乳腺癌模型组, 但无显著性差异。
      结论   麦胚黄酮类粗提取物具有较强的抗氧化活性, 可能是其对乳腺肿瘤呈现抑制作用的机理之一。

     

    Abstract:
      Objective   To investigate whether the flavonoids crude extrct in wheat germ could inhibite the mammary tumor induced by 7, 12-dimethlben z〔a〕anthracene and clarify the possible inhibitory machenism.
      Methods   Seventy female Sprague-Dawley(SD)rats aged 50 days were randomly divided into four groups: control group(10), mammary tumour group(20), low dose experimental group(20)and high dose experimental group(20).Except of rats in control group, each rat in other groups was given 15 mg 7, 12-dimethylben z〔a〕 anthracene(DMBA)dissolved in 1.5ml plantoil for twice every other two week by gavage.While the rats in low and high dose experimental groups were given forage contained of 2% and 10% flavonoids crude extract of wheat germ respectively, rats in control and mammary tumor groups were given basic forage.After 24 weeks administered DMBA to rats, the glutathione peroxidase(GSH-Px), superoxide dismutase(SOD), glutathione Stransferase(GST)activity and glutathion(GSH), malondialdehyde(MDA)level were measured respectively in blood and liver of rats.
      Results   The flavonoids crude extract could enhance the GSH-Px, SOD, GST activity and GSH level in blood and liver of rats, decrease the MDA level(P < 0.05).
      Conclusion   The flavonoids crude extracthad the anti-oxidation, which demonstrated one of mechanisms of chemophylactic effects on mammary tumor.

     

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