VES抑制Raji细胞增殖中对CDK2和Bcl-2/Bax蛋白表达的影响

肖浩文; 叶建锋; 欧程山; 高宁

VES抑制Raji细胞增殖中对CDK2和Bcl-2/Bax蛋白表达的影响

Changes of proteins expressions of CDK2、Bcl-2 and Bax in vitamin E succinate-induced growth inhibition in Raji cells

摘要

摘要:
目的比较观察维生素E琥珀酸酯(VES)和维生素E(VE)对人单核细胞白血病Raji细胞的抗增殖作用及对细胞周期素依赖性激酶2(CDK2), 细胞凋亡相关蛋白Bcl-2、Bax的影响。
方法应用荧光显微镜、流式细胞仪、免疫细胞化学染色等技术方法, 体外观察VES和VE对Raji细胞的作用。
结果 VES和VE对Raji细胞均有程度不一的生长抑制作用, 20μg/mlVES对Raji细胞的生长抑制在72h即达100%, 而20μg/mlVE的抑制率仅与5μg/mlVES的接近。VES较强的生长抑制作用与诱发细胞凋亡的明显增加同步发生, 具有剂量效应和时间效应关系, 同时VES作用后, 细胞内CDK2蛋白, Bcl-2蛋白表达下降, Bax蛋白表达增加。
结论 VES通过影响细胞周期调控因子CDK2, 细胞凋亡相关蛋白Bcl-2、Bax的表达可能是其抗肿瘤的途径之一。

Abstract:
Objective To investigate the effects of vitamin E succinate(VES)and vitamin E(VE)on cell growth inhibition and protein expressions of cyclin-dependent kinase-2, apoptosis-related proteins-Bcl-2 and Bax.
Methods Fluorescence microscope, flow-cytometry and immunocytochemical staining were used to observe the effects of VES and VE on Raji cells in vitro.
Results Both VES and VE could inhibit Raji cells growth.VES was more effective than VE, which inhibited cells growth and induced apoptosis in dose-dependent and time-dependent manners.Cells treated with 20μg/ml VES for 72 hours were inhibited absolutely, and the cellular growth inhibitory rate of 20 μg/ml VE was just similar to that of 5 μg/ml VES.During growth inhibition induced by VES in Raji cells, the expressions of CDK2 and Bcl-2 were down-regulated, while the expression of Bax was increased.
Conclusion The changes of expressions of CDK2.Bcl-2 and Bax may be related to the mechanism of anticancer biological activities of VES.

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