铁对热暴露K<sub>562</sub>细胞活力及热应激蛋白的影响

铁对热暴露K₅₆₂细胞活力及热应激蛋白的影响

摘要:
目的观察微量元素铁对热暴露K₅₆₂细胞活力及热应激蛋白HSP70影响, 探讨铁发挥热保护作用的可能机理。
方法以K₅₆₂细胞为研究对象, 在普通培养基RPMI1640里分别添加了不同浓度的硫酸铁、柠檬酸铁配制成实验培养液, MTT法测定细胞活力, Tissieres方法测定热应激蛋白HSP70含量。
结果一定浓度的硫酸铁(100~400μmol/L)、柠檬酸铁(100~200μmol/L)能显着提高K₅₆₂细胞的热耐力, 且热应激蛋白(HSP70)的合成也明显增加。
结论硫酸铁、柠檬酸铁能显着提高K₅₆₂细胞的热耐力, 但它们作用浓度和效果不同, 其作用机理可能与热应激蛋白HSP70的合成增加有关。

Abstract:
Objective To study the effects of Fe on viability of heat-stressed K₅₆₂ cell, HSP 70 and its probable mechanisms.
Methods K₅₆₂ cell were cultured with RPMI-1640 medium added with Fe at variant concentrations, then the cell viabilities of K₅₆₂ and the contents HSP 70 were detected after heat exposure.
Results A certain concertration of Fe₂SO₄(100-400 μmol/L), critric acid iron(100-200 μmol/L)could enhance viabilities of heat-exposed K₅₆₂ cell and their synthesis of HSP 70.
Conclusions These findings suggested that Fe could significantly promote thermoresistance of K₅₆₂ cell, and some of their mechanisms might due to the enhancing synthesis of HSP 70.