镉对去卵巢大鼠钙代谢的影响

杨学斌; 申立军; 周袁芬; 金泰廙

镉对去卵巢大鼠钙代谢的影响

Effects of cadmium on calcium metablisms of ovariectomized rats

摘要

摘要:
目的研究镉对去卵巢大鼠钙代谢的影响。
方法将SD大鼠行人工去卵巢术, 分别经饮水给不同剂量(50, 100, 200mg/L)的镉染毒24周。分别于第6, 12, 18, 24周收集24h尿液、粪便。用石墨炉原子吸收法测定血、尿镉, 放射免疫法测定血清雌二醇、尿β₂微球蛋白, 同时测定粪、尿及骨钙含量, 计算粪钙及尿钙日排泄总量。
结果大鼠去卵巢后, 体内雌二醇明显下降。镉染毒后, 去卵巢大鼠粪钙及尿钙日排泄总量明显升高, 且呈剂量反应关系.200mg/L镉染毒组去卵巢大鼠的骨钙含量也显着性下降.同时镉染毒的去卵巢大鼠发生肾损害, 表现为尿β₂微球蛋白排泄增加。尿钙日排泄总量与β₂微球蛋白显着性正相关。
结论镉可导致去卵巢大鼠钙代谢损害, 肠钙及尿钙的排泄量增加, 使骨钙含量降低。

Abstract:
Objective To investigate effects of cadmium on calcium metabolisms of ovariectomized rats.
Methods Female Sprague-Dawley rats aged 6 months were ovariectomized.The rats were administrated with cadmium at the dose of 0, 50, 100 and 200 mg/L in drinking water for 24 weeks.Urine and feces of 24 hours were collected after 6, 12, 18 and 24 weeks, respectively.Cadmium concentration in urine, blood and bone were measured by an atomic absorption spectrophotometer.And urinary β₂-MG and serum estradiol were detected by RIA.The calcium contents in bone, urine and feces were assayed.
Results Compared to the sham operation group, estradiol significantly declined in the ovariectomized rats.After cadmium administration, the excretions of calcium in feces and urine increased significantly in a dose-response related pattern.And calcium contents of bone were significantly lower in the group exposed to 200 mg/L cadmium in drinking water than in the control group.Urinary β₂-MG was significantly higher in cadmium-exposed groups.Meanwhile urinary β₂-MG positively related to total excretions of calcium in 24 hours.
Conclusion The results indicated that cadmium could inhibit calcium metabolisms by increasing the excretions of calcium in feces and urine, which probably resulted in significant calcium declines in bone.

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