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钟才高, 张洪霞, 曾明, 刘新民. 汽车尾气诱导小鼠体内氧化损伤与遗传毒性的研究[J]. 中国公共卫生, 2003, 19(1): 31-33.
引用本文: 钟才高, 张洪霞, 曾明, 刘新民. 汽车尾气诱导小鼠体内氧化损伤与遗传毒性的研究[J]. 中国公共卫生, 2003, 19(1): 31-33.
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ZHONG Cai-gao, ZHANG Hong-xia, ZENG Ming, . Study on oxidative damages and genotoxicity in mice induced by automobile exhaust[J]. Chinese Journal of Public Health, 2003, 19(1): 31-33.
Citation: ZHONG Cai-gao, ZHANG Hong-xia, ZENG Ming, . Study on oxidative damages and genotoxicity in mice induced by automobile exhaust[J]. Chinese Journal of Public Health, 2003, 19(1): 31-33.
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汽车尾气诱导小鼠体内氧化损伤与遗传毒性的研究

Study on oxidative damages and genotoxicity in mice induced by automobile exhaust

    摘要
  • 摘要:
      目的   探讨汽车尾气导致小鼠体内氧化损伤与遗传毒性的关系。
      方法   将受试小鼠随机分为4组, 即对照组与汽车尾气1:10, 1:20, 1:30稀释度染毒组, 采用动式染毒装置, 使小鼠暴露不同稀释度汽车尾气, 每天暴露2h, 连续暴露5d, 测定受试组小鼠体内血、肝、肺、睾丸中活性氧簇(ROS)、丙二醛(MDA)含量以及超氧化物歧化酶(SOD)活性与微核率的变化。
      结果   染毒组小鼠血、肝、肺、睾丸ROS与肺、睾丸MDA含量和骨髓细胞微核率明显升高, 与对照组比较, 其差异均有显着性意义(P < 0.01), 其中小鼠骨髓细胞微核率呈现一定剂量-效应关系.血、肝、肺ROS含量与微核率呈明显相关关系。
      结论   汽车尾气可导致机体氧化与抗氧化系统平衡紊乱; 并能引起细胞染色体损伤.汽车尾气导致机体组织的氧化损伤可能在细胞遗传毒性作用中起重要作用。

     

    Abstract:
      Objective   To study the relationship between oxidative damages, and genotoxicity in mice by automobile exhaust.
      Methods   Mice were randomly divided into one control group and three exposure groups and were respectively exposed fresh air and different concentrations of automobile exhaust to hours each day for 5d in the device designed by investigator's laboratory.The concentrations of reactive oxygen species(ROS), malondialdehyde(MDA)and the activities of superoxide dismutase(SOD)in blood, liver, lung, testicle and micronucleus frequency in bone marrow cells were respectively determined.
      Results   The concentration of ROS in blood, liver, lung, testicle and MDA in lung, testicle of mice in automobile exhaust exposed groups were significantly higher than in negative control group(P < 0.01).Compared to negative control group, the micronucleus frequency in bone marrow polychromatic erythrocytes(PCE)in automobile exhaust exposed groups increased significantly(P < 0.01)and revealed dose effect relationship to a certain extent.There were correlative relationships between ROS in blood, liver, lung and the micronucleus frequency.
      Conclusion   Automobile exhausts could break the balance between oxidative system and anti oxidative system and damage chromosomes of cells.The cell oxidative damages induced by exposed automobile exhaust may play a key role in the effect of genotoxicity.

     

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