单核吞噬细胞应答伤寒沙门菌的实验研究

李铁民; 徐浩; 江崎孝行

单核吞噬细胞应答伤寒沙门菌的实验研究

Research on mechanisms of mononuclear phagocyte in response to Salmonella typhi strains

摘要

摘要:
目的研究单核吞噬细胞应答伤寒沙门菌的机理。
方法采用体外培养的PMA分化的THP-1细胞, 检测其内化和杀伤胞内伤寒沙门菌的活性, 以及伤寒沙门菌诱导PMA分化的THP-1细胞产生TNF-α和IL-12的情况。
结果 PMA分化的THP-1细胞内化和杀伤V_i阴性伤寒沙门菌的活性明显高于内化和杀伤V_i阳性伤寒沙门菌的活性。IFN-γ明显增强此活性.伤寒沙门菌诱导PMA分化的THP-1细胞可以产生一定量的TNF-α和IL-12, 然而, IFN-γ活化的PMA分化的THP-1细胞产生TNF-α和IL-12的量明显增加。
结论伤寒沙门菌在单核吞噬细胞中的存活可能与V_i抗原的存在有关; IFN-γ在调节机体细胞免疫, 防御伤寒沙门菌感染中起着重要的作用。

Abstract:
Objective To research the mechanisms of mononuclear phagocyte in response to Salmonella typhi strains.
Methods The activities of internalization and intracellular survival of S.typhi strains, as well as the production of TNF-A and IL-12 induction by S.typhi strains were detected by using PMA differentiated THP-1 cells cultured in vitro.
Results Vi negative S.typhi strains were internalized and killed significantly by PMA differentiated THP-1 cells by contrast to V_i positive S.typhi strains.The activities of PMA differentiated THP-1 cells werer enhanced dramatically by IFN-C.The results also showed that the production of TNF-A and IL-12 can be induced by S.typhi strains in PMA differentiated THP-1 cells.However, the productive amount of TNF-A and IL-12 was significantly increased in PMA differentiaed THP-1 cells activated by IFN-C.
Conclusion The survial of S.typhi strains inside mononuclear phagocytes might be related to the presence of V_i antigen.Moreover, IFN-C played an important role in the regulation of the cellular immunity of immune system against S.typhi infection.

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