骨髓间充质干细胞诱导抗砷细胞

Study on arsenic toleranc induced by bone marrow mesenchymal stem cells

摘要:
目的利用低剂量NaAsO₂诱导骨髓间充质干细胞MSCs, 获得抗砷细胞, 探讨抗砷机制.
方法用1mol/L NaAsO₂对MSCs进行低剂量慢性诱导, 利用四甲基偶氮噻唑蓝法(MTT)计算细胞生存率及半数致死量, 观察细胞抗砷性的产生.
结果 NaAsO₂诱导18周后, 人骨髓MSCs产生稳定抗砷性, 砷诱导组细胞LC₅₀为25.8mol/L, 同步对照组细胞LC₅₀为11.4mol/L, 砷诱导组细胞LC₅₀是同步对照组细胞LC₅₀的2.2倍.
结论低剂量NaAsO₂长期诱导人骨髓MSCs, 可使细胞获得抗砷性.

Abstract:
Objective To obtain stable arsenic tolerance in bone marrow mesenchymal stem cells(MSCs).
Methods Bone marrow mesenchymal stem cells were exposed atlow level arsenite.The cell surviral rate and LC₅₀ were measured by MTT.The resistance to arsenite was observed.
Results Bone mar rowmesenchymal stem cells exposed to 1mol/L sodium arsenite for 18 weeks exhibited dramatic resistance to acute arsenite toxicity.The LC₅₀ for acute arsenite exposure in these chronic arsenic-esposed cells was 25.8mol/L versus 11.4mol/L in control cells.
Conclusion Acquisition of stable arsenic toler ance in bone mar row mesenchmal stem cells was available.