硒与铬对糖尿病小鼠糖代谢及抗氧化影响

张娅婕; 甘振威; 刘华锋; 徐大伟; 谢林

硒与铬对糖尿病小鼠糖代谢及抗氧化影响

Effects of selenium and chromium on glucose metabolism and antioxidant activity of diabetic mice

摘要

摘要:
目的观察硒、铬对实验性糖尿病小鼠糖代谢和抗氧化作用的影响.
方法选取健康雄性昆明种小鼠, 按200 mg/kg剂量左下腹腔内1次性注射四氧嘧啶, 制成糖尿病小鼠模型, 分别按每天80和40g/kg灌服亚硒酸钠和三氯化铬(Se⁴⁺和Cr³⁺), 同时设正常对照组和模型对照组.记录各组每天饮水与食量, 隔天称量体重1次, 观察4周后禁食24 h, 眼眶采血, 测定血糖及肝组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力及总抗氧化能力(T-AOC)、丙二醛(MDA)水平.
结果与模型对照组比较, 硒、铬干预组的血糖水平均明显降低(P < 0.01), SOD、GSH-Px活性及T-AOC水平均明显升高(P < 0.05或P < 0.01), MDA水平明显降低(P < 0.05或P < 0.01).
结论硒、铬具有降低实验性糖尿病小鼠血糖, 增强其抗氧化能力的作用.

Abstract:
Objective To study the effects of selenium and chromium on glucose metabolism and antioxidantactivity of diabetic mice.
Methods The model of experimental diabetic mice was established by abdominal cavity injection of alloxan with the dose of 200 mg/kg.The mice were divided into 4 groups:selenium, chromium, normal control and experimental control group.Selenium and chromium gr oup were respectively administered orally with the dose of 0.08 mg/(kgd)(Se⁴⁺)and 40 g/(kgd)(Cr ³⁺).After four weeks, blood glucose, T-AOC, M alondia ldehyde(MDA), Superoxide dismutas(SOD), G lutathione peroxidase(GSH-PX)were detected.
Results Compared to experimental group, blood glucose level of selenium and chromium group decreased significantly(P < 0.01), the activity of SOD and GSH-Px as well as T-AOC capacity increased obviously(P < 0.05 or P < 0.01).Meanwhile, the level of MDA declined remarkably(P < 0.05 or P < 0.01).
Conclusion Selenium and chromium can decrease the blood glucose, and can improve the antioxidantactivity of diabetic mice.

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