烟酰胺对紫外线诱发DNA损伤的保护作用

Protective effect of nicotinamide on ultraviolet radiation-induced DNA damage

摘要:
目的探讨烟酰胺对紫外线照射诱发DNA损伤的保护作用.
方法提取人外周血中的淋巴细胞, 采用单细胞凝胶电泳法观察经不同浓度烟酰胺处理并用紫外线照射所诱发的淋巴细胞DNA损伤状况.
结果随着紫外线照射剂量的增加, 淋巴细胞的DNA损伤程度逐渐加重; 在0.01~0.1 mmol/L浓度范围内, 外源性烟酰胺可明显减轻紫外线照射所诱发的DNA损伤, 其保护作用具有一定的剂量依赖趋势.
结论适宜浓度的烟酰胺是抗紫外线照射诱发DNA损伤的拮抗剂.

Abstract:
Objective To investigate the protective effectof nicotinamide on UVR-induced DNA damage.
Methods Afterextracting human peripheral blood lymphocytes and treating them with nico tinamide as well as ultraviolet radiation, the damag ed deg ree of lymphocy tic DNA was examined with single cell gel electrophoresis.
Results With the UVR dose increasing, the damage degree of lymphocytic DNA increased gr adually.After treating with nicotinamide within 0.01~0.1 mmol/L, UVR-induced DNA damage was reduced.The protective effectof nicotinamide showed dose-dependent manner.
Conclusion nicotinamide is a perfect antagonistto UVR-induced DNA damage within fitting concentration.