血清HCVRNA荧光定量与丙型肝炎诊断意义

Significance of fluorescent quantitative serum HCVRNA and diagnoses in hepatitis C

摘要:
目的探讨血清HCVRNA荧光定量在丙型肝炎诊断中的意义.
方法采用ELISA法和荧光定量PCR法检测292例慢性丙肝患者抗-HCV和HCVRNA.
结果抗-HCV及HCVRNA检出率分别为71.9%(210/292)和51.3%(150/292);抗HCV(+)组和抗-HCV(-)组HCVRNA的检出率分别为75.9%(82/108)和10.5%(4/83).
结论抗HCV(+)组HCVRNA检出率高于抗-HCV(-)组; 血清HCVRNA荧光定量是临床诊断丙型肝炎的直接证据; 抗-HCV诊断丙肝感染有漏诊现象.

Abstract:
Objective To explore the significance of serum levels of HCVRNA in hepatitis Cdiagnoses.
Methods The anti-HCV was monitored by indirectenzy me-linked immunosorbent assay (ELISA) and serum levels of HCVRNA quantities were tested by fluorescent quantitative PCR(FQ-PCR)in 292 cases of chronic hepatitis Cpatients.
Results The positive rates of anti-HCV and HCVRNA were 71.9%(210/292)and 51.3%(150/292), respectively.The rates of HCVRNA in positive anti-HCV group and negative group were 75.9%(82/108)and 10.5%(4/38), respectively.
Conclusion The monitored rate of HCVRNA in positive anti-HCV group patients is higher than thatof negative group.Serum levels of HCVRNA by FQ-PCR quantities are one of the bestdirectmar ks in diagnoses for gepatitis Cthatcould make up for the missed diagnoses with the mark of anti-HCV.