Sj26基因转染树突状细胞抗血吸虫感染作用

Protective immunity effect of Sj26 gene transfer dendritic cells on Schistosoma japonicum infection

摘要:
目的探讨日本血吸虫Sj 26基因转染树突状细胞(DC)抗血吸虫感染保护性免疫作用.
方法利用Sj26基因转染、空质粒pcDNA 3转染和未处理DC分别免疫BALB/c小鼠3次, 攻击感染后计数成虫和虫卵.
结果 Sj26基因转染DC免疫小鼠诱导34.5%的减虫率和48.5%的减卵率, 明显高于空质粒pcDNA 3转染DC组(16.8%和15.8%)和未处理DC组(14.6%和14.4%), 空质粒pcDNA 3转染DC和未处理DC组之间差异无统计学意义.
结论 Sj 26基因转染DC可诱导抗血吸虫感染的保护性免疫作用.

Abstract:
Objective To explore the effect of Sj 26 gene transfer dendritic cells(DC)in inducing protective immunity againstinfection of Schistosoma japonicum.
Methods BALB/c mice were immunized three times with Sj26 gene transfer DC, plasmid pcDAN 3 transfer DC and untreated DC, respectively.The number of adultworm and the egg number of liver were calculated six weeks after challenging with cercariae of Schistosoma japonicum.
Results The rate of worm reduction and egg reduction in the group of Sj26 transfer DC were 34.5% and 48.5% respectively, sig nificantly higher than that of group of plasmid pcDNA 3 transfer DC(16.8% and 15.8%)and untreated DC(14.6% and 14.4%).There was no significant difference between gr oup of plasmid pcDNA 3 transfer DC and group of untreated DC.
Conclusion Sj 26 gene transfer DC could induce protective immunity against infection of Schistosoma japonicum.