诊断丙型肝炎病毒感染的3种ELISA的比较研究

The Comparison Hepatitis C Virus of Three ELISA Systems for Daignosis of Infertion

摘要: 将新建立的检测血清中抗丙型肝炎病毒（HCV）总抗体的双抗原夹心ELISA（夹心法）与普遍采用的检测抗HCV-IgG的间接ELISA（IgG间接法）及检测抗HCV-IgG的间接ELISA（IgG间接法）进行比较研究。检测15份IgG间接法阳性、IgG间接法阴性（IgG单阳）血清及20份IgG间接法阳性、IgG间接法阴性（IgG单阳）血清，夹心法均为阳性，这35份血清经PCR检测HCVRNA均阳性。夹心法可同时检出IgG和IgG抗体，检出率高于后两者。检测20份HCVRNA阴性血清，3种方法均阴性。用生物制品检定所检测HCV－IgG抗体的质控血清为标准品，夹心法和IgG间接法均可达到检测标准。

Abstract: An antigen sandw iched ELISA (AS ELIS) detEcting talantibodies to hepatitis C Virus (HCV) was compared with widely applyed indirect EL1SA testing an ti-HCV-IgG(IgG ID ELISA) and an ti HIV-IgM ID ELISA) All of 15 IgG negative with IgM positive sera and 20 IgG and positive with IgM negative sera were positive for ASELISA. AS ELISA may detect bo th IgG and IgM ，the positive rate are higher than both IgG ID ELISA and IgM ID ELISA. T he re sults also showed that the sensitifisities and spesifisities of AS ELISA and IgG ID ELISA are similar (sensitivty ≥95%，spesifisity ≥95% general coincidentrate ≥95%)w hen detected apanel containing 198 quality cxm trol sera provided