Abstract: In order to study the acute toxicities of MT BE. Two MTBE samples(made in China and German) were studied The acutetoxicities were conducted with mice, rats or rabbits, through oral inhalation or demal cnntam inations Resultsshow th at inmice, LD₅₀ of China and German samples are 2945.2 and 2944. 4mg/kg inrats both are3433. 6mg/kg LC₅₀ of mice 72988.3 and 99630mg/kg and LC₅₀ of rats are 8530 and d103410mg/m³ respectively. Clinical symptoms of acute to toxicities include sedation, ocular and mucousmem brane irrition ataxia hypoactivity and ansthesicaetc There no irritation or toxicity to be find throughdennal cnntan inatpn But there is a significan tirritating to the rabb its eyes Conclution But thare are no sinkficant differences about acute toxicities between China and GeananMTBE samples Both belong to a lower toxiity substance through oralor inhalatpn cxmtan inations anda silight toxiity subtance through dermal Bud both have a significant irrritating to rabbits eyes.