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汪金凤, 俞永新. 流行性乙型脑炎病毒在恒河猴肾传代细胞上的蚀斑技术研究及其应用[J]. 中国公共卫生, 1986, 5(5): 55-59.
引用本文: 汪金凤, 俞永新. 流行性乙型脑炎病毒在恒河猴肾传代细胞上的蚀斑技术研究及其应用[J]. 中国公共卫生, 1986, 5(5): 55-59.
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Wang Jinfeng, Yu yongxin. Studies on Plaque Formation Assay on Continuons Macaca Kidney Cell Line with Japanese Encephalitis Virus and Its Application[J]. Chinese Journal of Public Health, 1986, 5(5): 55-59.
Citation: Wang Jinfeng, Yu yongxin. Studies on Plaque Formation Assay on Continuons Macaca Kidney Cell Line with Japanese Encephalitis Virus and Its Application[J]. Chinese Journal of Public Health, 1986, 5(5): 55-59.
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流行性乙型脑炎病毒在恒河猴肾传代细胞上的蚀斑技术研究及其应用

Studies on Plaque Formation Assay on Continuons Macaca Kidney Cell Line with Japanese Encephalitis Virus and Its Application

    摘要
  • 摘要: 流行性乙型脑炎（以下简称乙脑）病毒在原代鸡胚细胞和原代地鼠肾细胞上可以形成空斑。但因原代细胞来源比较困难，制备适合空斑形成的致密单层细胞难度较大，形成的蚀斑维持时间短。

     

    Abstract: Plaque formation assay was performed on continuous macaca kidney cell line (LLC-MK2) with Japanese B encephalitis virus (IBEV). Under experimental conditions, the increase in number of plaques stopped eight days after inoculation of the virus, thus the plaques could be counted.These strains of JBEV of high and low virulence, which had been passaged through mouse brains, were compared and showed no difference in plaque formation.

     

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