大肠菌不耐热肠毒素ELISA与Y-1细胞试验的对比研究

Comparative Study of ELISA and Y-1 Cell Test for Detection of Thermo-labile Enterotoxin of Escherichia coli

摘要: 自1977年Yolken等建立了测定大肠菌不耐热肠毒素(LT)的酶联免疫吸附试验(ELISA)后，Svennerholm等又利用GMI—神经节苷酯作为LT的捕捉剂，建立了GMI—ELISA。随后Sack等又加以改进。1983年Czerkinsky等又建立了在塑料平皿上直接用菌落进行的GMI—酶联免疫色斑试验。

Abstract: By taking advantage of presence of cross imm unoreaction between thermo-labile enterotoxin of Escherichia coli(LT) and cholera toxin (CT), anti CT-ELISA was developed for detection of LT and was compared with the Y- 1 cell test. The rate of coincidence in detection of 68 strains of Escherichia coli by these two methods 97% and the P/N value of anti CT-ELISA was closely with the intensity of Y-1 cell reaction(r=0.88).