Abstract: Ovariectomy has a proven pronounced effect on osteoporosis.In the present study ,ovariectomized felmal Wistar rate(170±15g) were treated with vitamin Kor/and calcium for 6 months,in order to study the effect of vitaminK or/and calcium supplementation on the prevention of osteoporosis.VitaminK(90mg/kg) or/and calcium(5g/kg)were added to the regular diet(Vk50μg/kg,Ca5g/kg).The findings were as follows:Vitamin K supplementaton reduced urinary calcium and hydroxyproline excretion,suggesting a decrease in bone resorption;At the same time,vitamin K elevated the circulating levels of osteocalcin and OC bound,suggesting an incrdease in bone mineralization.And more,the effect of vitamin K combined with calcium on the prevention of osteoporsis is stronger than that of vitamin K or calcium solely.The present study indicated that postmenopausa women,as a risk popular of osteoporosis,should increase their their intakes of vitamin K and calcium.Vitamin K may be used as a pharmacology to prevent and cure osteoporosis.