Abstract: This method refers to the International Standard ISO9308-1:1990,Water quality-Detection and enumeration of coliform organisms,thermotolerant coliform organisms and presumptive Escherichia coli.Using the newest method of membrane filtration for coliform organisms in water.The media in this method is used first in China.So we have made inquiry to the electivity and speciality of the media,Tergitol and TTC in order to see if they have the effect on the enumeration of coliform organisms.The experiment result shows that on the elective media using in this method the negative bacterium shows gree and the positive shows yellow.So the elective media's electively and speciality are good.Within the scope of ISO method,the media without Tergitol has no effect on the enumeration of coliform organisms.TTC has no effect on the enumeration of coliform or ganisms when it's dose is in the scope of ISO method.