烟溶液与温石棉对人胚肺细胞芳香烃羟化酶活性的影响
Effect of Chrysotile and Cigarette Smoke Solution on Activity of Aryl Hydrocarbon Hydroxylase Separately or Simulhaneously in Human Embryo Lung Cells
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摘要: 为探讨吸烟与温石棉在致癌过程中的联合作用,用已建株的人胚肺(HEL)细胞在体外测定了烟溶液与温石棉单独及联合作用对细胞内芳香烃羟化酶((AHH)活性的影响?结果表明:烟溶液在剂量间虽也有一定的梯度变化,但在统计学上无显著性意义,而温石棉则可明显地诱导HEL细胞内AHH的活性升高,且有剂量-反应关系;当烟溶液与温石棉联合作用时对细胞AHH活性的升高呈明显的相加作用,提示在吸烟与温石棉的联合作用过程中,温石棉可通过诱导AHH活性升高,促使烟中的多环芳烃更易代谢为终致癌物,从而导致肺癌的发生?Abstract: In order to investigate the combind effect of chrysotile and cigarette smoking on carcinogenesis,activity of aryl hydrocarbon hydroxylase(AHH) induced by chrysotile and cigarette smoke solution separately or simultaneously were studied in Human Embryo Lung(HEL) cell lines invitro.The results showed that chrysotile could include the increase of AHH activity in HEL cells and adductive effect could be seen were exposed to chrysotile and cigarette smoking solufion simultaneously, suggesting that chrysotile may exert its cocaminogenic effects by enhancing AHH activity in cells exposed to dgarette smoke, and make it easier for cells to transform PAH to ultimate carcinogens.
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